| Field | Specification |
|---|---|
| Target | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Recombinant fusion protein of human DLAT (NP_001922.2). |
| Conjugate | |
| Purification | |
| Concentration | |
| Storage | |
| Storage buffer | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood.
Product Overview
This polyclonal antibody, raised in Rabbit, recognizes DLAT. It is validated for Western Blot, Immunohistochemistry, Immunofluorescence.
Validated Applications
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence |
|---|---|
| WB | 1:500-1:2000 |
| IHC | 1:50-1:100 |
| IF | 1:50-1:200 |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
Recombinant fusion protein of human DLAT (NP_001922.2).
Specifications
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Recombinant fusion protein of human DLAT (NP_001922.2). |
| Purification | Affinity Purification |
| Concentration | 1 mg/mL |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3 |
| Calculated MW | 68kDa |
| Observed MW | 69kDa |
| UniProt # | P10515 |
| Lead Time | 2-3 business days |
| Validity | 12 months |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice packs |
| Target Synonyms | DLAT;DLTA;PDC-E2;PDCE2 |
Reactivity Notes
Validated reactivity: Human, Mouse, Rat. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for DLAT research from Elabscience Biotechnology Co Ltd, or contact us for alternative formats.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
