DLD Antibody, FITC conjugated

Overview

This polyclonal anti-DLD antibody raised in Rabbit, conjugated with FITC. Designed to detect DLD in Human for signal transduction research.

Key elements and design rationale

• Immunogen: Recombinant Human Dihydrolipoyl dehydrogenase, mitochondrial protein (398-495AA) — determines epitope; confirm sample prep compatibility.
• Host (Rabbit): Requires anti-rabbit-IgG secondary matching isotype IgG.
• Polyclonal: Multiple epitopes = robust signal; lot-to-lot variability — include controls.
• Isotype (IgG): Match secondary, Protein A/G, and isotype controls to IgG.
• Purification (Protein G purification): Enriches Ig classes; reduces non-specific background.
• FITC: Direct fluorescence (Ex490/Em525); protect from light; use antifade.

Biological background

DLD (also: Dehydrogenase complex, E3 component, Diaphorase, Dihydrolipoamide dehydrogenase, Dihydrolipoyl dehydrogenase, Dihydrolipoyl dehydrogenase mitochondrial, dld, DLDD, DLDH, DLDH_HUMAN, E3, E3 branched chain aplha-keto acid, E3 component of pyruvate dehydrogenase, E3 component of pyruvate dehydrogenase complex 2 oxo glutarate complex branched chain keto acid dehydrogenase complex, GCSL, Glycine cleavage system L protein, Glycine cleavage system protein L, LAD, lipoamide dehydrogenase, Lipoamide reductase, Lipoyl dehydrogenase, mitochondrial, OTTHUMP00000206744, OTTHUMP00000206746, OTTHUMP00000206748, OTTHUMP00000206749, PHE 3, PHE3, Pyruvate dehydrogenase component E3) — studied in Human systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current DLD annotation relevant to signal transduction.

Common research applications

• Refer to product documentation for confirmed application data.

Notes for experimental interpretation

• Isotype controls: IgG from Rabbit at equivalent concentration.
• Cross-reactivity: Polyclonal: validate with KO/KD controls.
• Matrix effects: Verify dilution linearity in your sample matrix.
• Reactivity: Confirmed for Human; extrapolation requires validation.

This product is a polyclonal antibody purified from rabbit antiserum.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.