DMS-79 cell

SKU:BHC11100541
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Overview
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DMS-79 cell is a cell line derived from Caucasian (Male). It is commonly used as an in vitro model for 1 research. Growth characteristics: Aggregates in suspension. Supplied as cryopreserved cells with accompanying batch CoA and quality-control documentation.

Species Human
Disease model Carcinoma, azaserine induced
Growth Properties Aggregates in suspension
Tissue Lung
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Catalog no. Size
300164 1 cryovial
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This cell line is available in the U.S. For non-profit users, please sign and submit the Non-Profit Supply Agreement to orders@biohippo.com before placing an order. For commercial users, please complete the CLEAR Form before ordering, as additional usage fees may apply based on the intended use. For further details, please contact orders@biohippo.com. Products ship after the required agreement is completed; typical delivery is 2–3 business days. Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10^6 cells for adherent lines or 5 × 10^6 cells for suspension lines (refer to the batch CoA for details).

Field Specification
Mfr No 300164
Species Human
DMS-79 is a human lung cancer cell line derived from a small cell lung carcinoma. These cells exhibit a classical neuroendocrine phenotype, which is characteristic of small cell lung cancer. This phenotype is significant because it implies a potential utility in studying neuroendocrine signaling pathways, which are crucial in the development and progression of lung cancer. The DMS-79 cell line has been widely utilized in research to understand the molecular biology of lung cancers, particularly in the context of tumor genesis, cell proliferation, and apoptosis. The cell line is known for its aggressive growth and high tumorigenicity in vivo, making it an excellent model for in vivo studies of tumor behavior and response to therapeutics. DMS-79 cells also serve as a useful tool for pharmacological testing and drug development, offering insights into the cellular responses to various chemotherapeutic agents. Furthermore, these cells have been instrumental in the study of cancer stem cell characteristics and mechanisms of metastasis in small cell lung carcinoma. This extensive usage underscores the importance of DMS-79 in cancer research, particularly in therapies targeting aggressive and hard-to-treat cancers like small cell lung carcinoma.

SKU:BHC11100541

  • Receptors expressed: Epidermal growth factor (EGF)
  • Antigen expression: Leu 7, My23, Class 1 HLA, Class 2 HLA
  • Oncogenes: c-myc +, N-myc +, c-raf-1 +, Ha-ras +, Ki-ras +, N-ras +, v-fes -, v-fms -
  • Tumorigenic: Yes, in nude mice
  • Products: Adrenocorticotropin (adrenocorticotropic hormone, ACTH), bombesin, calcitonin, corticotropin, beta endorphin, 17 beta estradiol, lipotropin, oxytocin - neurophysin (OT-NP), parathormone, somatostatin-like immunoreactivity (SRIF)
  • cultureMedium: RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
  • supplements: Supplement the medium with 10% heat-inactivated FBS, add 2.5 g/L glucose and 10 mM HEPES
  • doublingTime: 96 hours
  • subculturing: Once or twice a week add 5 ml of fresh cell culture medium, as soon as the culture medium gets acidic. Subculture as soon as many very large clusters are observed. Dissociate the clusters by collecting the cells, rinsing once using PBS without calcium/magnesium and adding 3-5 ml Accutase. Incubate at 37 degree Celsius for 10minutes. Collect the cells following centrifugation, resuspend in fresh cell culture medium and count. Start cultures at 2-4 x 104 cells/ml.
  • seedingDensity: 2 to 4 x 104 cells/cm2
  • fluidRenewal: 2 to 3 times per week
  • postThawRecovery: After thawing, allow the cells to recover from the freezing process for at least 24 hours.
  • freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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