| Field | Specification |
|---|---|
| Product Type | |
| Shipping | |
| Source | Recombinant (E. coli) |
| Storage |
DNA Polymerase I, in the presence of template and primer (DNA or RNA), uses dNTP as a substrate to synthesize DNA complementary to the template in the 5'→3' direction. The molecular weight of this enzyme is about 109,000 Da, and it has double-strand-specific 5'→3' exonuclease activity and single-strand-specific 3'→5' exonuclease activity.
Applications
With DNase I (Cat#10607) for nick translation reaction
With RNase H (Cat#12906) to synthesize the second strand of cDNA
Specifications
|
Concentration |
10 U/μL |
|
Unit Definition |
One unit is defined as the amount of enzyme required to degrade 1 µg of circular plasmid within a 10-minute period at 37°C in a 100 µl reaction system |
Glycerol Content |
Contains Glycerol |
Components
|
Components No. |
Name |
12903ES60 |
12903ES76 |
12903ES92 |
|
12903-A |
DNA polymerase I (10 U/μL) |
50 μL |
250 μL |
1 mL |
|
12903-B |
10×DNA polymerase I Reaction Buffer |
1 mL |
1×5 mL |
4×5 mL |
Shipping and Storage
This product should be stored at -25 ~ -15℃ for 2 years.
Store this enzyme at -20°C and avoid repeated freeze-thaw cycles to preserve catalytic activity. The product is shipped Dry Ice and remains stable for up to one year from the date of manufacture when stored under recommended conditions. Aliquoting the stock solution into single-use volumes is recommended for enzymes used infrequently to minimize thermal cycling of the bulk stock.
With DNase I (Cat#10607) for nick translation reaction With RNase H (Cat#12906) to synthesize the second strand of cDNA. Always verify compatibility with your specific template, buffer, and downstream workflow.
One unit (U) is defined as the amount of enzyme that catalyzes the incorporation of 10 nmol of deoxyribonucleotide into acid-insoluble material in 30 min under standard assay conditions.
This enzyme is produced as Recombinant (E. coli) and supplied as a Research Use Only (RUO) reagent. Each lot is subjected to activity assay, purity assessment by SDS-PAGE, and functional validation prior to release. A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) are available on request.
This enzyme is produced recombinantly in E. coli under stringent quality controls and validated on multiple substrate types. Key performance parameters include specific activity, substrate specificity, cofactor requirements, optimal pH/temperature range, and compatibility with downstream assays. Refer to the product datasheet for validated lot-specific activity data and recommended reaction conditions.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
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