| Field | Specification |
|---|---|
| Applications | |
| Source | Recombinant (E. coli) |
| Storage | |
| Shelf life | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Deoxyribonuclease I (DNase I) is a recombinant DNase I protein expressed in E. coli, without RNase. Hydrolysis of single- or double-stranded DNA leaves a phosphate group at the 5' end and a hydroxyl group at the 3' end. DNase I activity is dependent on calcium ions and can be activated by magnesium ions or divalent manganese ions. In the presence of magnesium ions, DNase I can randomly cleave any site of double-stranded DNA; in the presence of divalent manganese ions, DNase I can cleave DNA double-strands at the same site to form blunt ends or short sticky ends with 1–2 nucleotide overhangs.
The enzyme is supplied as a single reagent rather than a kit, in packs of 1000 U (500 µL) and 2000 U (1 mL). Documented uses are removal of DNA contamination from RNA samples, elimination of DNA template after transcription, nick translation, DNase I footprinting for protein–DNA interaction studies, generation of random DNA fragment libraries and use as a positive control in apoptosis TUNEL assays.
Specifications
| Format | Single reagent, recombinant DNase I solution |
|---|---|
| Source | Recombinant (E. coli) |
| RNase content | Without RNase |
| Substrate | Single- or double-stranded DNA |
| Cofactor requirement | Activity is dependent on calcium ions |
| Activation by Mg²⁺ | Random cleavage at any site of double-stranded DNA |
| Activation by Mn²⁺ | Cleavage of both strands at the same site, giving blunt ends or 1–2 nucleotide overhangs |
| Product ends | 5' phosphate group and 3' hydroxyl group |
Applications
- Removal of DNA contamination from RNA samples and of DNA template after transcription
- Nick translation
- DNase I footprinting for protein–DNA interaction studies
- Generation of random DNA fragment libraries
- Positive control in apoptosis TUNEL assays
Key Features
- No cumbersome phenol/chloroform extraction or inactivation is required after digestion.
- The digested sample can be used directly in a reverse transcription reaction, which is fast and easy to use.
Storage & Handling
Stored at room temperature with a shelf life of one year.
Safety & Regulatory
For research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.
Customization & Add-ons: Need this molecular biology reagent tailored to your workflow? We can help with bulk or custom pack sizes, alternative formats (spin column, magnetic bead or reagent-only kits; premixed or stand-alone reference dye), matched extraction, cDNA synthesis and qPCR workflows, and sourcing of related enzymes, ladders and consumables not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
