| Field | Specification |
|---|---|
| Mfr No | |
| Product Type |
DNase I is a non-specific endonuclease derived from bovine pancreas that catalyzes the cleavage of phosphodiester bonds in single/double-stranded DNA, chromatin, and RNA:DNA hybrids to generate di-and/or oligonucleotide (5’-phosphorylated and 3’-hydroxylated) end-products.
| Product Component | Quantity |
|---|---|
| DNase I | 2,000 U (1.0 ml) |
| 10X DNase I Reaction Buffer | 1.0 ml |
| Specification | Value |
|---|---|
| Enzyme Type | DNase |
| Format | Enzyme Only |
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DNase I requires divalent cations for activity. Mg²⁺ (2–5 mM) promotes double-stranded DNA cleavage producing nicks; Mn²⁺ produces double-strand breaks. EDTA inactivates the enzyme by chelating these cations.
Inactivate by heat treatment at 75°C for 10 minutes in the presence of 2.5 mM EDTA, or remove via spin column purification. Avoid phenol-chloroform extraction, which may cause RNA degradation.
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