| Field | Specification |
|---|---|
| Mfr No | |
| Product Type |
DNase I is a non-specific endonuclease derived from bovine pancreas that catalyzes the cleavage of phosphodiester bonds in single/double-stranded DNA, chromatin, and RNA:DNA hybrids to generate di-and/or oligonucleotide (5’-phosphorylated and 3’-hydroxylated) end-products. This product is RNase-free and can be used in RNA applications.
| Product Component | Quantity |
|---|---|
| DNase I (RNase-Free) | 200 U (100 μl) |
| 10X DNase I Reaction Buffer | 1.0 ml |
| Specification | Value |
|---|---|
| Enzyme Type | DNase |
| Format | Enzyme Only |
DNase I requires divalent cations for activity. Mg²⁺ (2–5 mM) promotes double-stranded DNA cleavage producing nicks; Mn²⁺ produces double-strand breaks. EDTA inactivates the enzyme by chelating these cations.
Inactivate by heat treatment at 75°C for 10 minutes in the presence of 2.5 mM EDTA, or remove via spin column purification. Avoid phenol-chloroform extraction, which may cause RNA degradation.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
References
- Tang X; Khan H; Niewola-Staszkowska K et al.. Inhibition of autotaxin activity with IOA-289 decreases fibrosis in mouse E0771 breast tumors. International journal of cancer. 2025;157:1205-1217. DOI
