| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A portion of amino acids 14-43 from the human protein was used as the immunogen for this DNASE1L3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
DNASE1L3 Antibody / Deoxyribonuclease gamma is an antibody targeting DNASE1L3, raised in Rabbit for protein detection and localization studies where these specifications are required.
Key elements and design rationale
- Target: DNASE1L3.
- Antibody identity: Polyclonal (rabbit origin); Rabbit Ig.
- Conjugate/label: Unconjugated (affects detection chemistry and multiplex compatibility).
- Format: Antigen affinity purified.
- Species reactivity: Rat.
- Listed applications: WB (refer to on-page specifications for application-specific guidance).
Biological background
DNASE1L3 is a member of the deoxyribonuclease enzyme family, responsible for breaking down DNA molecules into smaller fragments. This process is essential for the clearance of cellular debris, including dying cells and extracellular DNA released during inflammation. Without the action of DNASE1L3, the accumulation of DNA waste can lead to autoimmune diseases and other inflammatory conditions. Research has shown that DNASE1L3 plays a key role in modulating immune responses and protecting against inflammatory damage. By efficiently clearing DNA debris, this enzyme helps to maintain the balance between immunity and tolerance in the body. Dysregulation of DNASE1L3 activity has been linked to various autoimmune disorders, highlighting the importance of this enzyme in immune homeostasis. Furthermore, DNASE1L3 has been identified as a potential therapeutic target for treating autoimmune diseases and inflammatory conditions. By enhancing the activity of this enzyme, researchers hope to restore immune balance and prevent the development of autoimmune reactions.
Research relevance and current trends
- Comparative expression profiling across cell types, tissues, or perturbations (e.g., drug treatment, genetic editing, or differentiation).
- Subcellular localization and trafficking studies, including co-localization with pathway markers in microscopy-based assays.
- Integration of protein-level measurements with transcriptomics or proteomics to relate abundance to regulation and phenotype.
Common research applications
- Western blotting: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
Interpretation should account for antibody-dependent factors such as epitope accessibility, isoforms, and sample preparation differences across workflows.
Notes for experimental interpretation
- Isoforms and PTMs: many targets have multiple isoforms and post-translational modifications that can shift apparent signal or localization; interpret bands/signals accordingly.
- Epitope context: binding can depend on protein conformation and sample processing; region information in the title/immunogen can help anticipate what may be detected.
- Species differences: predicted or validated reactivity may vary by ortholog sequence and sample context; confirm in your model system.
- Control concepts: include negative controls (no-primary/isotype), and where possible genetic controls (KO/KD) or independent antibodies to strengthen conclusions.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
