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Product Overview
This kit uses a unique buffer system and is especially suitable for extracting genomic DNA from fresh plant materials. It is safe and convenient to use. It can remove impurities, proteins and other organic compounds in cells to the greatest extent. This product does not contain harmful chemical components such as phenol, chloroform and so on. It is safer for the operator. The kit yields high-molecular-weight genomic DNA of high purity. The DNA recovered by this kit can be used in various routine operations, including enzyme digestion, PCR, library construction, Southern hybridization and so on.
Plant tissue of 100 mg is ground under liquid nitrogen and lysed in 600 µL of Buffer PGE with 6 µL of RNase A at 60°C for 20 min. The cleared lysate is mixed with 0.3 volumes of absolute ethanol and loaded onto the adsorption column, washed with 500 µL of Solution RP and twice with 600 µL of Wash Buffer, air-dried for 5–10 min and eluted with 30–50 µL of Elution Buffer. Purified DNA has an OD260/OD280 of 1.7–1.9.
Specifications
| Format | Spin column |
|---|---|
| Sample type | Fresh plant material |
| Sample input | 100 mg fresh tissue or 20 mg dry weight |
| Yield | 3–30 µg genomic DNA from 100 mg plant tissue |
| Purity | OD260/OD280 should be 1.7–1.9 |
| Processing time | Within 1 h |
| Chemistry | Phenol/chloroform-free |
| Elution volume | 30–50 µL |
Kit Components
| Component | 50 T | 200 T |
|---|---|---|
| Buffer PGE | 35 mL | 140 mL |
| Wash Buffer | 60 mL | 240 mL |
| Solution RP | 30 mL | 120 mL |
| Elution Buffer | 2.5 mL | 10 mL |
| RNase A | 315 µL | 1.25 mL |
| Adsorption column | 50 set | 200 set |
Applications
- Genomic DNA extraction from fresh plant tissue
- Enzyme digestion and PCR
- Library construction
- Southern hybridization
Key Features
- Simple and fast: High purity genomic DNA can be obtained within 1 h.
- Safe and low toxicity: No need to use phenol/chloroform extraction, safe operation.
- High purity and good quality: 3–30 µg of genomic DNA can be extracted from 100 mg of plant tissue, and the value of OD260/OD280 is in the range of 1.7–1.9.
Storage & Handling
Buffer PGE, Wash Buffer, Solution RP, Elution Buffer and the adsorption columns are stored at room temperature for 1 year. RNase A is stored at -20°C for 1 year.
Usage Notes
- Avoid repeated freeze–thaw cycles.
- Pre-dissolve precipitated Buffer PGE at 56°C before use.
- Use fresh plant tissue.
- Add absolute ethanol to the Wash Buffer before first use, as instructed in the manual.
- Air-dry the column for 5–10 min at room temperature before elution.
- The dissolved volume affects both yield and final concentration.
Safety & Regulatory
For research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.
Customization & Add-ons: Need this molecular biology reagent tailored to your workflow? We can help with bulk or custom pack sizes, alternative formats (spin column, magnetic bead or reagent-only kits; premixed or stand-alone reference dye), matched extraction, cDNA synthesis and qPCR workflows, and sourcing of related enzymes, ladders and consumables not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
