| Field | Specification |
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| Biosafety level | |
| Product format | Frozen |
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Overview
Dog Primary CD3+ Pan T Cells are dog frozen primary cells from peripheral blood used for T-cell activation, signaling, proliferation, and immune response studies. These cells display suspension, round growth characteristics. Donor history reported for this listing: Young Adult Male.
Key elements and design rationale
- Biological source: Dog (Canine)
- Tissue origin: Peripheral Blood
- Growth properties: Suspension, round
- Format: Frozen
- Donor history: Young Adult Male
- Biosafety level: II
- Culture context: PriGrow X Series Medium (TM5495) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂.
Primary T cells are central to adaptive immune responses and are commonly studied for activation-state transitions, cytokine signaling, proliferation, and cell–cell communication.
Research relevance and current trends
- Primary T-cell assays frequently evaluate activation markers, cytokine production, and proliferation under defined stimuli.
- Subset-specific or enriched T-cell preparations support mechanistic studies of signaling and donor heterogeneity.
- Co-culture and conditioned-media systems are often used to model cross-talk with antigen-presenting or stromal cells.
Common research applications
- Assess activation, proliferation, and cytokine responses after stimulation.
- Use in immune co-culture assays to study cell–cell signaling and response modulation.
- Profile donor- or condition-dependent changes in phenotype and functional readouts.
Product-specific data supplied for this listing
- Growth Conditions: PriGrow X Series Medium (TM5495) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂.
- Warranty: abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period”.
- Disclaimer: 1. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at orders@biohippo.com.
Notes for experimental interpretation
- Primary cells can show donor-dependent and passage-dependent variation, so morphology, growth rate, and marker expression should be interpreted in the context of the specific lot and culture history.
- Attachment matrix, medium formulation, and gas conditions can materially influence phenotype maintenance and experimental readouts; use the recommended culture system where possible.
- Cryopreserved recovery conditions matter for viability and downstream behavior. We recommend using serum-free CryoGuard™ Freezing Media (TM078) or, if serum is preferred, Cryopreservation Medium (TM024).
- Thaw cells quickly in a 37°C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination.
- Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.
- Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 125xg for 5-7 minutes.
- Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask.
- Incubate the cells at the recommended conditions.
- Simply add fresh complete media directly to the culture. Do not allow cell density to exceed 1x10⁶ cells/ml.
- Alternatively, replace complete growth media by centrifugation and re-suspend the cell pellet in fresh complete media, and add appropriate aliquots of the cell suspension to new culture vessels, as desired.
- Incubate the cells at the recommended conditions.
Cell line sourcing and selection (species, tissue, and disease model matching) · Stable cell line engineering (overexpression, knockdown, knockout via CRISPR/Cas9, shRNA, sgRNA) · Reporter gene integration (GFP, RFP, luciferase, fluorescent/bioluminescent constructs) · Genome editing and knockin (point mutations, tagged endogenous proteins, conditional alleles) · Inducible expression systems (Tet-On/Off and regulatable constructs) · Drug resistance marker selection (puromycin, G418, hygromycin, and others) · Custom growth and media optimisation for specific assay requirements · Scale-up production for high-throughput screening campaigns · Authentication and QC services (STR profiling, mycoplasma testing, viability assessment). Talk to a Scientist or contact support@biohippo.com.
