DOPC Nanodisc

What it means: 10 nm or 30 nm — the approximate diameter of the nanodiscs formed.

Why it matters:

• Nanodisc size limits which membrane proteins or complexes fit inside one disc.
• Larger discs can hold larger proteins or more lipid around them.
• Smaller discs give a more uniform particle for some structural studies.
• Size also affects how nanodiscs behave in purification and imaging.
• Choosing the size before you start avoids repeating the reconstitution.
• Disc size can be checked by dynamic light scattering or electron microscopy.
• Using one disc size throughout a study keeps conditions comparable.
• Disc size also affects how much lipid surrounds the reconstituted protein.

For this product: Choose 10 nm or 30 nm: 10 nm discs use SMA copolymer 3:1 and 30 nm discs use SMA copolymer 2:1.

Tip: Choose the larger disc for larger membrane proteins or complexes.

What it means: DOPC, cholesterol, DSPE-PEG2000 — the lipids that form the bilayer.

Why it matters:

• The PEGylated lipid adds a polymer layer at the disc surface.
• Lipid composition affects bilayer fluidity and the stability of reconstituted proteins.
• Cholesterol makes the bilayer more ordered and less permeable.
• Matching composition to your study makes results easier to compare with published work.
• Stating the composition makes your methods easy to report and repeat.
• The phase transition temperature of the main lipid affects how the bilayer behaves at assay temperature.
• Changing composition between experiments can change protein activity and stability.

For this product: The nanodisc lipids are DOPC, cholesterol, DSPE-PEG2000 at 5 mg/ml total.

Tip: Keep the pH at 7.0–8.0, the ideal range for SMA nanodiscs, and avoid high levels of divalent cations, which can precipitate SMA.

What it means: Cold, Not Frozen — how the product is shipped.

Why it matters:

• The shipping condition tells you what to expect when the package arrives.
• Unpacking promptly lets you check the contents against the order.
• Checking the package on arrival helps you spot transport problems early.
• Storage conditions can differ from shipping conditions, so confirm them before the package arrives.
• Recording the arrival date and condition helps if questions come up later.
• Arranging for someone to receive the package avoids delays when it arrives.
• The shipping condition is chosen to keep the product as described during transport.

For this product: Ships cold, not frozen. Please contact us to confirm storage conditions.

Tip: Do not freeze nanodiscs; check the package on arrival and contact us right away if anything is damaged or missing.