DOT1L Antibody

Overview

This polyclonal anti-DOT1L antibody raised in Rabbit, tested in ELISA, WB, IHC. Designed to detect DOT1L in Human for epigenetics and nuclear signaling research.

Key elements and design rationale

• Immunogen: Recombinant Human Histone-lysine N-methyltransferase, H3 lysine-79 specific protein (331-484AA) — determines epitope; confirm sample prep compatibility.
• Host (Rabbit): Requires anti-rabbit-IgG secondary matching isotype IgG.
• Polyclonal: Multiple epitopes = robust signal; lot-to-lot variability — include controls.
• Isotype (IgG): Match secondary, Protein A/G, and isotype controls to IgG.
• Purification (Protein G purification): Enriches Ig classes; reduces non-specific background.

Biological background

DOT1L (also: Disrupter of telomere silencing protein 1, DOT 1, DOT1, DOT1 like, DOT1 like histone H3 methyltransferase (S. cerevisiae), DOT1 like histone H3 methyltransferase, DOT1 like histone H3K79 methyltransferase, DOT1 like protein , DOT1, S. cerevisiae, homolog of, DOT1-like protein, DOT1L, DOT1L_HUMAN, EC 2.1.1.43, H3 K79 HMTase, H3 lysine-79 specific, H3-K79-HMTase, Histone H3 K79 methyltransferase, Histone H3 methyltransferase, Histone H3 methyltransferase DOT1-like, Histone H3-K79 methyltransferase, Histone lysine N methyltransferase H3 lysine 79 specific, Histone methyltransferase DOT1L, Histone-lysine N-methyltransferase, KIAA1814, KMT4, Lysine N methyltransferase 4, Lysine N-methyltransferase 4) — studied in Human systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current DOT1L annotation relevant to epigenetics and nuclear signaling.

Common research applications

• ELISA: Quantify soluble target; ensure samples within the linear detection range.
• WB: Confirms MW, distinguishes isoforms under denaturing conditions.
• IHC: Tissue expression in FFPE/frozen sections; optimize antigen retrieval.

Notes for experimental interpretation

• Isotype controls: IgG from Rabbit at equivalent concentration.
• Cross-reactivity: Polyclonal: validate with KO/KD controls.
• Matrix effects: Verify dilution linearity in your sample matrix.
• Reactivity: Confirmed for Human; extrapolation requires validation.

This product is a polyclonal antibody purified from rabbit antiserum.

• Datasheet (12554650)

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.