DPPC Liposome for DNA and RNA Nanoparticles

What it means: DPPC : cholesterol : dodecylamine 6.6 : 3.4 : 1 — the lipids that form the bilayer.

Why it matters:

• Lipid composition affects membrane rigidity, stability and drug release.
• PEGylated lipids add a polymer layer that changes how liposomes interact with proteins and cells.
• Cholesterol makes the bilayer more stable and less leaky.
• Matching composition to your study makes results easier to compare with published work.
• Stating the composition makes your methods easy to report and repeat.
• The phase transition temperature of the main lipid affects how the bilayer behaves at assay temperature.
• Changing composition between experiments can change results even with the same cargo.

For this product: The lipid mix is DPPC : cholesterol : dodecylamine 6.6 : 3.4 : 1; dodecylamine provides the positive charge.

Tip: Keep the lipid-to-nucleic acid ratio constant when comparing preparations.

What it means: 120 nm (empty) — the average diameter of the liposomes.

Why it matters:

• Liposome size affects how liposomes move through tissues and how they are taken up by cells.
• Size also affects how fast encapsulated material is released.
• A narrow size range makes results more reproducible.
• Knowing the size helps you choose filters or columns for purification.
• Stating the size makes your methods easy to report and repeat.
• Size can be checked by dynamic light scattering before use.
• Comparing size before and after an experiment shows whether liposomes stayed intact.
• Particle size also influences how liposomes scatter light in optical measurements.

For this product: Average size is 120 nm (empty); size almost doubles after loading at a lipid-to-nucleic acid mass ratio of 33.

Tip: Measure size after loading to confirm nanoparticle formation.

What it means: Cold, Not Frozen — how the product is shipped.

Why it matters:

• The shipping condition tells you what to expect when the package arrives.
• Unpacking promptly lets you check the contents against the order.
• Checking the package on arrival helps you spot transport problems early.
• Storage conditions can differ from shipping conditions, so confirm them before the package arrives.
• Recording the arrival date and condition helps if questions come up later.
• Arranging for someone to receive the package avoids delays when it arrives.
• The shipping condition is chosen to keep the product as described during transport.

For this product: Ships cold, not frozen. Storage: Store at 2–8°C. Do not freeze.

Tip: Do not freeze liposomes; check the package on arrival and contact us right away if anything is damaged or missing.