| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Shipping | |
| Storage |
Description
dsDNA BR Assay Kit is a simple, sensitive, accurate and wide range fluorescence quantification kit for double-stranded DNA (dsDNA BR) with good linearity in the range of 2~1000 ng. The kit contains fluorescence detection reagent, buffer and related dsDNA BR standards. Before use, the fluorescence detection reagent is diluted into working solution with buffer, then the dsDNA sample to be measured is added, a standard curve is made, and readings are performed using a fluorescent enzyme marker or Qubit® fluorometer. The product has good tolerance to conventional contaminants such as proteins and salts.
Features
- Good linearity: can accurately quantify 2~1000 ng double-stranded DNA (dsDNA BR);
- High specificity: good tolerance to common contaminants such as proteins, salts, etc.;
- Strict batch performance and stability quality control.
Components
|
Components No. |
Name |
Concentration |
12643ES60 |
12643ES76 |
|
12643-A |
dsDNA BR Reagent |
200×concentrate in DMSO |
250 μL |
1.25 mL |
|
12643-B |
dsDNA BR Buffer |
Not applicable |
50 mL |
250 mL |
|
12643-C |
dsDNA BR Standard 1 |
0 ng/μL in TE buffer |
1 mL |
5×1 mL |
|
12643-D |
dsDNA BR Standard 2 |
100 ng/μL in TE buffer |
1 mL |
5×1 mL |
Shipping and Storage
This product should be stored at 2~8℃ for six months.
Application
dsDNA Detection; DNA quantification
Figures
1. High detection sensitivity:
Figure 1. 0-1000 ng linear relationship
2. Tolerance to Common contaminants
Figure 2. Test results of tolerance to different contaminants
Prepare different types of contaminant solutions and mix each with an equal volume of S2 to create the test samples. Use various products to measure the concentration of each test sample, with an input volume of 10 μL per assay.
3. Working Solution Stability Test
Figure 3. Working Solution Stability Test
Documents:
Safety Data Sheet
Manuals
This kit requires a fluorometer capable of detecting the kit's excitation/emission wavelength (typically ~480/520 nm for dsDNA HS and similar dyes). The Qubit fluorometer (Thermo Fisher) or compatible fluorometer-based systems are recommended. Standard UV-Vis spectrophotometers using A260 cannot substitute for fluorometric quantification when sample purity or volume is limiting.
Sensitivity varies by kit format: the HS (High Sensitivity) kit detects dsDNA as low as 0.2 ng/µL and the BR (Broad Range) kit covers 2–1000 ng/µL. Refer to the specific product datasheet for the validated linear range. Samples exceeding the range must be diluted before measurement.
Yes. The HS and BR dsDNA kits contain intercalating dyes with strong preference for double-stranded DNA over RNA and ssDNA. This selectivity makes them suitable for quantifying genomic DNA, PCR products, and library preparations in the presence of residual RNA without a DNase or RNase pre-treatment step.
Yes. Fluorometric dsDNA quantification is the recommended method for NGS library quantification, as it measures only double-stranded library fragments and is unaffected by free adapter dimers or unincorporated nucleotides (unlike A260). For highest accuracy on sequencing libraries, combine with Bioanalyzer or TapeStation sizing.
Yeasen Biotechnology offers flexible customization options for many of its assay kits and detection reagents, including custom lot sizes, bulk ordering, and application-specific formulation adjustments. Volume pricing, custom packaging, and kit bundling may be available depending on the product and intended workflow. A Certificate of Analysis (CoA) and lot-specific QC data are provided with every order. For inquiries regarding large-volume orders, custom configurations, or integration into automated workflows, please contact the BioHippo team for a tailored quotation.
