| Field | Specification |
|---|---|
| Target | |
| Applications | |
| Assay type | |
| Sample type(s) | tissue |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
The Tribioscience dsRNA Fast ELISA is designed to quantitatively detect dsRNA levels in different tissues, cells, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate and fast. The measurement can be finished in 3 hours, not needing 4-5 hours (Fig. 1). The detection range is from 20 to 1280 pg/mL. The levels of dsRNA samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural dsRNA protein. The dsRNA Fast ELISA can be used to determine dsRNA in multiple-species.
Typical Data This standard curve (R 2 =0.9995) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity The minimum detectable dose (MDD) of dsRNA is typically 10 pg/mL.
dsRNA ELISA Kit is a Solid-phase sandwich ELISA for the quantitative determination of dsRNA. The microplate is pre-coated with a capture antibody specific to the target. Target present in the sample or standard binds the immobilized antibody, is detected with a biotinylated detection antibody and streptavidin-HRP, and quantified colorimetrically against a standard curve.
Kit Components
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. Refer to the product datasheet for the complete component list and quantities.
Performance Data
| Target | dsRNA |
|---|---|
| Sample Type | tissue |
| Assay Type | Sandwich |
| Test Principle | Solid-phase sandwich ELISA |
| Sensitivity | 10 pg/mL |
| Detection Range | 20–1280 pg/mL |
| Assay Time | 3 hours |
| Precision | Intra-assay CV 3.79%; Inter-assay CV 10% |
| Specificity | This assay recognizes natural and recombinant dsRNA. |
| Storage | Store the unopened kit at 2-8°C. |
✓ Quality tested for reproducibility and lot-to-lot consistency
Safety & Handling
Customization & Add-ons: Need this ELISA kit tailored to your workflow? We can help with bulk or custom pack sizes, species or analyte variants, matched antibody pairs and standards, alternate plate formats (48- or 96-well, strip-well), and assay development or custom ELISA solutions for targets not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
