Dylight 549, Rabbit Anti-Goat IgG

Reactivity: Goat

What it means: This secondary antibody is designed to recognize goat primary antibodies (e.g., goat IgG). It binds to the primary antibody and enables detection through its conjugate (enzyme or fluorophore).

Why it matters: Choose “Goat” reactivity when your primary antibody is raised in goat. Note that in goat-derived samples, endogenous goat immunoglobulins may contribute to background in certain applications.

Host: Rabbit

What it means: “Host” refers to the animal in which the secondary antibody was produced. Host: Rabbit means the antibody was generated by immunizing a rabbit, and the resulting rabbit antibodies were collected and purified to make this secondary antibody.

Why it matters: The host species can affect background and cross-reactivity. For best results, avoid using a rabbit-host secondary in workflows where rabbit immunoglobulins are present (e.g., rabbit primary antibodies in the same assay, rabbit serum-based blocking, or rabbit-derived samples), unless the protocol includes appropriate blocking and controls.

This secondary antibody is conjugated with DyLight 549, enabling direct fluorescent detection in the orange/red channel.

What it does: DyLight 549 is an orange/red fluorescent dye. After the DyLight 549-labeled secondary antibody binds to your primary antibody, you can visualize the target using a fluorescence microscope or imaging system with DyLight 549-compatible filters (typically excitation ~550–560 nm, emission ~570–590 nm).

Why it matters: DyLight 549-conjugated secondary antibodies are commonly used for immunofluorescence (IF) and other fluorescence-based assays, especially for multi-color staining with green- and far-red-channel fluorophores. It provides bright signal and good photostability for orange/red-channel imaging.

Application(s): IF, ICC, FCM

IF (Immunofluorescence): Used as a secondary antibody to bind the primary antibody and provide a fluorescent signal for target visualization under a fluorescence microscope (depending on the conjugate and filter set).

ICC (Immunocytochemistry): Used as a secondary antibody to detect primary antibodies bound to cellular targets in fixed cells, enabling fluorescent imaging of protein localization with the appropriate conjugate.

FCM (Flow Cytometry): Used as a secondary antibody to label primary antibody–bound cells for fluorescence-based detection and analysis by flow cytometry (matched to the instrument’s laser and emission channels).

Isotype: Rabbit IgG

What it means: This secondary antibody is a rabbit-derived immunoglobulin G (IgG). In other words, it is an IgG antibody produced in rabbit and purified for use as a secondary reagent.

Why it matters: “Rabbit IgG” is mainly useful for understanding the species origin and antibody class of the secondary antibody:

• Species origin (rabbit): This tells you the secondary itself is made in rabbit. If your sample contains rabbit immunoglobulins (e.g., rabbit serum/plasma, rabbit tissue, or you are working in rabbit models), endogenous rabbit IgG can contribute to background. In such cases, additional blocking or an alternative secondary/detection strategy may be needed.
• Antibody class (IgG): IgG is the most commonly used antibody class for secondary reagents and typically provides strong, reliable performance in routine detection workflows.
• Detection and capture compatibility: Many detection/capture reagents are designed to bind IgG (e.g., anti-rabbit IgG reagents and, in some workflows, Protein A/G). Knowing it is rabbit IgG helps confirm compatibility when extra capture/detection steps are used.
• Controls: If you are using anti-rabbit detection reagents, “rabbit IgG” helps when selecting appropriate negative controls to evaluate non-specific binding from rabbit-derived antibodies.