E. coli D-Ala-D-Ala Ligase

What it means: Cell Wall Synthesis Enzymes — enzymes of the bacterial peptidoglycan (cell wall) pathway.

Why it matters:

• These enzymes build the peptidoglycan layer that bacteria need to survive.
• Enzymes of the same pathway pass products to one another, so they can be studied side by side.
• The group helps you find related enzymes and assay kits in the catalog.
• Many enzymes of this pathway are essential, which makes them attractive antibacterial drug targets.
• Knowing the pathway step helps you choose the right substrates and controls.
• Studying several steps of the pathway helps you see where an inhibitor acts.

For this product: Cell Wall Synthesis Enzymes — joins two D-Ala molecules into the D-Ala-D-Ala dipeptide used to build peptidoglycan.

Tip: Use the ProFoldin protocol “E. coli D-Alanine D-Alanine Ligase Assay” (under Documents) as a starting point for reaction conditions.

What it means: E. coli — the organism the enzyme or protein comes from.

Why it matters:

• Enzymes from different organisms can differ in activity and in inhibitor sensitivity.
• Using the organism of your study keeps results relevant to that organism.
• Comparing the same enzyme from several organisms shows how selective an inhibitor is.
• The organism helps you choose the matching assay kit and controls.
• Stating the organism makes your methods easy to report and repeat.
• Results from one organism do not always carry over to the matching enzyme of another organism.

For this product: Use this E. coli enzyme when your study or inhibitor panel targets E. coli.

Tip: Use the ProFoldin protocol “E. coli D-Alanine D-Alanine Ligase Assay” (under Documents) as a starting point for reaction conditions.

What it means: 200 nM — the concentration of the supplied stock.

Why it matters:

• The stock concentration decides how far you dilute the enzyme for each reaction.
• Knowing it helps you plan how many reactions one vial supports.
• Keeping the same final enzyme amount in every well keeps results comparable.
• It lets you compare your conditions with the matching kit protocol.
• A known stock value makes dilution steps easy to document.
• Keep the final enzyme concentration the same when you change the reaction volume, and check the linear range for your conditions.

For this product: Each vial holds 33 µl of 200 nM E. coli D-Ala:D-Ala ligase.

Tip: Dilute just before use, keep the enzyme on ice, and prepare only the amount needed for the day.

What it means: Frozen — how the product is shipped.

Why it matters:

• Frozen shipping keeps the product cold during transport.
• Knowing the shipping condition helps you arrange for someone to receive and store the package.
• The product should be moved to cold storage as soon as it arrives.
• Checking the package on arrival helps you spot transport problems early.
• Planning storage space in advance avoids delays on delivery day.
• Frozen products should not be left at room temperature after delivery.

For this product: Frozen — the vial is shipped frozen; move it to cold storage as soon as it arrives.

Tip: Check the package on arrival, move the vial to cold storage right away, and contact us if it is not frozen.