| Field | Specification |
|---|---|
| Product Type | |
| Shipping | |
| Source | Recombinant (E. coli) |
| Storage |
E. coli DNA ligase can catalyze the formation of phosphodiester bonds between the 5’-PO4 ends and 3’-OH ends of adjacent DNA strands. It exhibits almost no detectable activity for blunt-end substrate DNA. The optimal pH for this reaction is 7.5-8.0 (Tris-HCl buffer), and NAD is required as a coenzyme. Unlike T4 DNA Ligase, which can join both sticky and blunt ends, this enzyme only catalyzes the ligation between cohesive-ended DNA molecules. However, in the presence of PEG and high concentrations of monovalent cations, it can also ligate blunt-ended DNA. Additionally, this product cannot catalyze the ligations between DNA and RNA or between RNA molecules.This enzyme can be used for ligation reactions in NGS methylation library and can also be employed in the Okayama-Berg method for cDNA cloning.
Specifications
|
Concentration |
60 U/μL |
|
Unit Definition |
In a 20 μL ligation reaction system, the amount of enzyme required to ligate over 90% of 6 μg of λ DNA-Hind III fragments at 16°C for 30 minutes is defined as one unit of activity (U) |
Glycerol Content |
Contains Glycerol |
Components
|
Name |
14955ES82 |
|
|
14955-A |
E. coli DNA ligase(60 U/μL) |
20 μL |
|
14955-B |
10× E. coli DNA ligase Buffer |
100 μL |
|
14955-C |
10× BSA(0.05%) |
100 μL |
Note: BSA should be protected from repeated freezing and thawing. For short-term storage, it can be kept at 4℃.
Shipping and Storage
The products should be stored at -25℃ ~ -15℃ for 1 year.
Documents:
Safety Data Sheet
Manuals
Store this enzyme at -20°C and avoid repeated freeze-thaw cycles to preserve catalytic activity. The product is shipped Dry Ice and remains stable for up to one year from the date of manufacture when stored under recommended conditions. Aliquoting the stock solution into single-use volumes is recommended for enzymes used infrequently to minimize thermal cycling of the bulk stock.
This enzyme is validated for use in Cloning & Assembly, NGS Library Preparation applications. It is suitable for use in both standard laboratory research settings and, where applicable, optimized reaction workflows requiring high specificity, sensitivity, or throughput.
One unit (U) is defined as the amount of enzyme that catalyzes the ligation of greater than 95% of HindIII-linearized lambda DNA fragments into high-molecular-weight concatemers in 30 min at 16°C in a 20 µL reaction volume.
This enzyme is produced as Recombinant (E. coli) and supplied as a Research Use Only (RUO) reagent. Each lot is subjected to activity assay, purity assessment by SDS-PAGE, and functional validation prior to release. A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) are available on request.
Standard T4 DNA ligase requires overnight ligation at 16°C for optimal efficiency with cohesive ends. Quick Ligase formulations contain optimized buffer systems and enhanced enzyme concentrations that achieve >90% ligation efficiency within 5–15 minutes at room temperature, significantly accelerating cloning workflows without compromising junction fidelity.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
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