E. coli MurF Assay Kit Plus

SKU:BHT16300040
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E. coli MurF Assay Kit Plus for measuring MurF activity and screening antibacterial inhibitors, with a phosphate detection dye readout in 384-well plate format. UDP-MurNAc-tripeptide, D-Ala-D-Ala and ATP substrates and E. coli MurF enzyme included; available in 100-assay and 500-assay sizes.
Assay Target E. coli MurF activity
Plate Format 384-well plate
Options selector
Catalog no. Size
MURF100KE 100 assays
MURF500KE 500 assays
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 100 assays, 500 assays
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: Please contact us to confirm storage conditions.
  • Shipping: Shipped frozen.
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Organism E. coli
Applications
  • Enzyme Activity
  • Compound Profiling
Assay type
  • Enzyme Activity Assay
  • Inhibitor Screening Assay
Assay target E. coli MurF activity
Assay area Antibacterial Drug Discovery
Target type Ligase
Plate format 384-well plate
Storage Please contact us to confirm storage conditions.
Shipping Shipped frozen.
Catalog no. (Mfr.) MURF100KE | MURF500KE
Main SKU BHT16300040
Drug Target Assay Kit

E. coli MurF is a bacterial cell wall synthesis enzyme and a target for antibacterial drug discovery.

Pack Sizes

Both sizes include the E. coli MurF enzyme. The only difference is the number of assays: MURF100KE is for 100 assays and MURF500KE for 500 assays (384-well format); see the component table for the volumes of each size.

100 assays (MURF100KE) 100 assays; enzyme included
500 assays (MURF500KE) 500 assays; enzyme included

Kit Components

Component 100 assays (MURF100KE) 500 assays (MURF500KE)
10 x Buffer 400 µl 2 ml
100 x UDP-MurNAc-tripeptide (UMAG-DAP) 35 µl 170 µl
100 x D-Ala-D-Ala 35 µl 170 µl
100 x ATP 35 µl 170 µl
1000 x E. coli MurF (5000 nM) 10 µl 20 µl
Phosphate detection dye 5 ml 25 ml

Specifications

Assay Target E. coli MurF activity
Target Type Ligase
Organism E. coli
Assay Type Enzyme Activity Assay; Inhibitor Screening Assay
Plate Format 384-well plate
Sizes 100 assays, 500 assays
Storage Please contact us to confirm storage conditions.
Shipping Shipped frozen.

Protocol

A detailed protocol is not available online for this kit. Please contact us and we will request the instructions from the manufacturer for you.

Safety & Handling

For Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. The E. coli MurF Assay Kit Plus is shipped frozen. Follow standard laboratory safety practices when handling the reagents. Please contact us to confirm storage conditions.
Q.What does this kit measure?
A.It measures the activity of E. coli MurF, a bacterial cell wall synthesis enzyme, using a dye for phosphate detection. It can be used to test compounds against this antibacterial target.
Q.What is the difference between the options?
A.Both sizes include the E. coli MurF enzyme. The only difference is the number of assays: MURF100KE is for 100 assays and MURF500KE for 500 assays (384-well format); see the component table for the volumes of each size.
Q.Is a protocol available?
A.A detailed protocol is not available online for this kit. Please contact us and we will request the instructions from the manufacturer for you.
Q.How should the kit be stored?
A.The E. coli MurF Assay Kit Plus is shipped frozen. Please contact us for long-term storage conditions.

Can’t find the exact format you need? We can help you source or customize this E. coli MurF activity assay — including larger pack sizes, bulk reagents, bulk enzyme, or a protocol adapted to your samples. Click Talk to a Scientist, email support@biohippo.com, or explore our Research Services. Our team will be in touch shortly.

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Try Celltrypse Free – Request Your Sample Today

Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

Try Celltrypse Free – Request Your Sample Today