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E. coli MurF is a bacterial cell wall synthesis enzyme and a target for antibacterial drug discovery.
Pack Sizes
Both sizes include the E. coli MurF enzyme. The only difference is the number of assays: MURF100KE is for 100 assays and MURF500KE for 500 assays (384-well format); see the component table for the volumes of each size.
| 100 assays (MURF100KE) | 100 assays; enzyme included |
|---|---|
| 500 assays (MURF500KE) | 500 assays; enzyme included |
Kit Components
| Component | 100 assays (MURF100KE) | 500 assays (MURF500KE) |
|---|---|---|
| 10 x Buffer | 400 µl | 2 ml |
| 100 x UDP-MurNAc-tripeptide (UMAG-DAP) | 35 µl | 170 µl |
| 100 x D-Ala-D-Ala | 35 µl | 170 µl |
| 100 x ATP | 35 µl | 170 µl |
| 1000 x E. coli MurF (5000 nM) | 10 µl | 20 µl |
| Phosphate detection dye | 5 ml | 25 ml |
Specifications
| Assay Target | E. coli MurF activity |
|---|---|
| Target Type | Ligase |
| Organism | E. coli |
| Assay Type | Enzyme Activity Assay; Inhibitor Screening Assay |
| Plate Format | 384-well plate |
| Sizes | 100 assays, 500 assays |
| Storage | Please contact us to confirm storage conditions. |
| Shipping | Shipped frozen. |
Protocol
A detailed protocol is not available online for this kit. Please contact us and we will request the instructions from the manufacturer for you.
Safety & Handling
Can’t find the exact format you need? We can help you source or customize this E. coli MurF activity assay — including larger pack sizes, bulk reagents, bulk enzyme, or a protocol adapted to your samples. Click Talk to a Scientist, email support@biohippo.com, or explore our Research Services. Our team will be in touch shortly.