| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Shipping | |
| Source | Recombinant (E. coli) |
| Storage |
E.coli Poly(A) polymerase, also known as Poly A tail-adding enzyme, catalyzes the addition of AMP converted from ATP to the 3 'end of single-stranded RNA in A template-independent manner to form Poly(A) tail. E.coli Poly(A) polymerase can use a variety of single-stranded RNA as a substrate, but DNA can not be used as a substrate. Double-stranded RNA and short oligonucleotides are not recommended as substrates. The Poly(A) tailing reaction catalyzed by E.coli Poly(A) Polymerase can only use ATP, but not ADP or dATP. The incorporation of CTP and UTP was less than 5% of that of ATP. When GTP is used, it cannot be added to the 3 'end of the RNA at all.
Features
When applied to miRNA detection, it demonstrates enhanced sensitivity.
No residual nuclease or RNase.
Applications
RNA Modification.
miRNA Expression Analysis.
cDNA Synthesis & Reverse Transcriptases Products.
RNA Synthesis In vitro Transcription (IVT).
Specifications
|
Unit Definition |
One unit is defined as the amount of enzyme that will incorporate 1 nmol of AMP into RNA in a 20 µl volume in 10 minutes at 37oC |
|
Purity |
≥90% |
Glycerol Content |
Contains Glycerol |
Components
|
Components No. |
Name |
14801ES60 |
14801ES76 |
|
14801-A |
E.coli poly(A) Polymerase (5 U/μL） |
20 μL |
100 μL |
|
14801-B |
10×Reaction Buffer |
100 μL |
500 μL |
Shipping and Storage
This product should be stored at -25 ~ -15oC for 2 years.
Store this enzyme at -20°C and avoid repeated freeze-thaw cycles to preserve catalytic activity. The product is shipped Dry Ice and remains stable for up to one year from the date of manufacture when stored under recommended conditions. Aliquoting the stock solution into single-use volumes is recommended for enzymes used infrequently to minimize thermal cycling of the bulk stock.
RNA Modification . miRNA E xpression Analysis . cDNA Synthesis & Reverse Transcriptases Products . RNA Synthesis In vitro Transcription (IVT) .. Always verify compatibility with your specific template, buffer, and downstream workflow.
One unit (U) is defined as the amount of enzyme that catalyzes the incorporation of 10 nmol of deoxyribonucleotide into acid-insoluble material in 30 min under standard assay conditions.
This enzyme is produced as Recombinant (E. coli) and supplied as a Research Use Only (RUO) reagent. Each lot is subjected to activity assay, purity assessment by SDS-PAGE, and functional validation prior to release. A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) are available on request.
This enzyme is produced recombinantly in E. coli under stringent quality controls and validated on multiple substrate types. Key performance parameters include specific activity, substrate specificity, cofactor requirements, optimal pH/temperature range, and compatibility with downstream assays. Refer to the product datasheet for validated lot-specific activity data and recommended reaction conditions.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
