| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | BFP; EBFP; Blue Fluorescent Protein; Enhanced Blue Fluorescent Protein |
| Clonality | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Immunogen | Recombinant Protein |
| Isotype | |
| Product Type | |
| Reactivity | |
| Source | This product is a polyclonal antibody purified from mouse antiserum. |
| Storage | |
| Target | |
| UniProt # |
Overview
This is a polyclonal anti-RNF112 antibody raised in Mouse, with confirmed utility in WB, IHC, IP, IF, ELISA. It is designed to detect RNF112 protein in Species Independent and supports researchers working in epigenetics and nuclear signaling contexts.
Key elements and design rationale
- Immunogen: Recombinant Protein — determines the epitope region; confirm compatibility with sample preparation and expected post-translational modifications.
- Host species (Mouse): Requires anti-mouse-IgG secondary reagents for indirect detection.
- Polyclonal format: Recognizes multiple epitopes for robust signal; inherent lot-to-lot variability requires appropriate controls.
- Isotype (IgG): Matched secondary antibodies and isotype controls required. Compatible with standard Protein A/G purification workflows.
- Purification (Antigen affinity purification): Enriches for specific immunoglobulin classes; reduces non-specific background vs. crude antisera.
Biological background
RNF112 (also referred to as BFP, EBFP, Blue Fluorescent Protein, Enhanced Blue Fluorescent Protein) is a protein target studied in Species Independent systems. Expression, subcellular localization, and post-translational modifications vary across cell types and disease states — factors critical to antibody-based detection design. Consult UniProt, NCBI Gene, and primary literature for current annotation of RNF112 biology in epigenetics and nuclear signaling.
Common research applications
- WB: Confirms target molecular weight and distinguishes isoforms under denaturing conditions.
- IHC: Visualization of target in FFPE or frozen tissue sections. Optimize antigen retrieval and secondary detection for each tissue type.
- IP: Enrichment of target-containing complexes from lysates for co-IP or proteomics.
- IF: Subcellular localization in fixed cells or tissue sections; fixation and permeabilization conditions affect epitope accessibility.
- ELISA: Quantification of soluble target in biological fluids or culture supernatants. Ensure samples are within the linear detection range.
Notes for experimental interpretation
- Isotype controls: Use an isotype-matched (IgG from Mouse) control at equivalent concentration to assess non-specific background.
- Cross-reactivity: Polyclonal preparations may cross-react with related proteins. Orthogonal validation (siRNA, KO lysate) is recommended.
- Matrix effects: Sample matrix can affect performance; pilot dilution linearity and spike-recovery experiments are advised for quantitative studies.
- Species reactivity: Confirmed for Species Independent. Extrapolation to untested species requires empirical validation.
This product is a polyclonal antibody purified from mouse antiserum.
RNF112 is a protein target in Species Independent biology. This polyclonal antibody raised in Mouse is designed to detect RNF112 in WB, IHC, IP, IF, ELISA applications, with IgG isotype.
Reported reactive against Species Independent. Immunogen derived from Species Independent. Cross-reactivity with other species should not be assumed without documented data or empirical testing.
Reported for WB, IHC, IP, IF, ELISA. Each format requires independent dilution optimization and appropriate controls. Unlisted applications require empirical testing.
This non-conjugated IgG antibody requires a compatible secondary. Select anti-mouse IgG secondary conjugated to your preferred reporter (HRP, AP, fluorophore, or biotin), matched to IgG.
(1) Isotype control — IgG from Mouse at matching concentration; (2) Positive control — known RNF112-expressing cell/tissue; (3) Negative control — knockdown/knockout sample for specificity; (4) Dilution linearity — verify proportional signal decrease in your matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
