| Field | Specification |
|---|---|
| Target | |
| Alternative names | SPI-1005; PZ-51; CCG-39161 |
| CAS no. | |
| Applications | |
| Molecular weight | |
| Molecular formula | C13H9NOSe |
| Purity | |
| SMILES | |
| Form | Solid |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
Ebselen, also known as SPI-1005, PZ-51, or CCG-39161, is a glutathione peroxidase mimetic and a potent blocker of voltage-dependent calcium channels (VDCC)[1][2]. It potently inhibits Mpro (IC50 = 0.67 μM) and the COVID-19 virus (EC50 = 4.67 μM)[3]. It also inhibits HIV-1 capsid CTD dimerization. As an organoselenium compound, it can permeate the blood-brain barrier and has anti-inflammatory, antioxidant, and anticancer activity[4][5]. It is supplied as an off-white to light yellow solid (C13H9NOSe, MW 274.18) at 99.80% purity.
Physical & Chemical Properties
| CAS Number | 60940-34-3 |
|---|---|
| Molecular Formula | C13H9NOSe |
| Molecular Weight | 274.18 g/mol |
| Purity | 99.80% |
| Appearance | Solid |
| Color | Off-white to light yellow |
| SMILES | O=C1N(C2=CC=CC=C2)[Se]C3=C1C=CC=C3 |
| Target | HIV-1 |
| Signaling Pathway | Neuronal Signaling; Membrane Transporter/Ion Channel; Anti-infection; Metabolic Enzyme/Protease |
| Solubility | In Vitro: DMSO: 50 mg/mL (182.36 mM; Requires sonication; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) |
| Storage | Powder: -20°C, 3 years; 4°C, 2 years. In solvent: -80°C, 1 year; -20°C, 6 months. |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
[2]. H Sies, et al. Ebselen, a Selenoorganic Compound as Glutathione Peroxidase Mimic
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with the Safety Data Sheet and your institution's chemical hygiene plan.
In Vitro
| Solvent | Solubility | Notes |
|---|---|---|
| DMSO | 50 mg/mL (182.36 mM) | requires sonication; use freshly opened DMSO (absorbed moisture lowers solubility) |
Aliquot the stock solution and store it at -80°C (up to 1 year) or -20°C (up to 6 months); avoid repeated freeze-thaw cycles.
In Vivo
Choose the formulation that suits the animal model and route of administration; percentages are volume ratios of the final working solution. Start from a clear DMSO stock (see In Vitro above), add the co-solvents one at a time in the order listed, mixing after each addition, and prepare the working solution fresh on the day of dosing. If precipitation or phase separation occurs, gentle warming or sonication can help.
Protocol 1
| Composition | 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% saline |
|---|---|
| Result | ≥ 2.5 mg/mL (9.12 mM); clear solution |
| How to prepare | Gives a clear solution at ≥ 2.5 mg/mL (saturation not determined). For 1 mL of working solution: add 100 μL DMSO stock (25.0 mg/mL) to 400 μL PEG300; then 50 μL Tween-80; then 450 μL saline to bring the volume to 1 mL. Saline: dissolve 0.9 g sodium chloride in ddH2O and make up to 100 mL. |
Protocol 2
| Composition | 10% DMSO + 90% Corn Oil |
|---|---|
| Result | ≥ 2.5 mg/mL (9.12 mM); clear solution |
| How to prepare | Gives a clear solution at ≥ 2.5 mg/mL (saturation not determined). Use with caution if continuous dosing will exceed two weeks. For 1 mL of working solution: add 100 μL DMSO stock (25.0 mg/mL) to 900 μL corn oil. |
Data provided by the manufacturer.
In Vitro
Ebselen (SPI-1005; 0.4-100 μM; 20-24 hours) shows strong antiviral effects at 10 μM in COVID-19 virus infected Vero cells. Ebselen covalently binds C145 of the catalytic dyad in COVID-19 virus Mpro[3]. In the HIV-1 life cycle, Ebselen inhibits early viral postentry events by impairing uncoating of the incoming capsid[4]. In mouse brain, Ebselen crosses the blood-brain barrier and inhibits endogenous inositol monophosphatase. Ebselen also inhibits IMPase (inositol monophosphatase)[5]. QSOX1 enzymatic activity is inhibited by Ebselen, which also suppresses invasion of pancreatic and renal cancer cell lines[6].
RT-PCR[3]
| Cell Line | COVID-19 virus infected Vero cells |
|---|---|
| Concentration | 0.4, 1.2, 3.7, 11.1, 33.3, 100 μM |
| Incubation Time | 20-24 hours |
| Result | Showed strong antiviral effects at a concentration of 10 μM treatment. |
In Vivo
Ebselen (5, 10 mg/kg; IP) reduces 5-HT2 agonist-induced head twitches in a dose-dependent manner[5].
| Animal Model | 20-25 g 10-12 week old male C57Bl6 mice[5] |
|---|---|
| Dosage | 5, 10 mg/kg |
| Administration | IP |
| Result | Decreased 5-HT2 agonist-induced head twitches in a dose-dependent manner. |
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
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