| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | GFP; Green Fluorescent Protein; enhanced Green Fluorescent Protein; |
| Clonality | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Immunogen | Purified recombinant EYFP.tag full length expressed in E.coli. |
| Isotype | |
| Product Type | |
| Reactivity | |
| Source | This product is a monoclonal antibody produced from mouse hybridoma cells. |
| Storage | |
| Target | |
| UniProt # |
Overview
This is a monoclonal anti-EGFP/EYFP antibody raised in Mouse, with confirmed utility in ELISA, WB. It is designed to detect EGFP/EYFP protein in Bacillus cereus and supports researchers working in others contexts.
Key elements and design rationale
- Immunogen: Purified recombinant EYFP.tag full length expressed in E.coli. — determines the epitope region; confirm compatibility with sample preparation and expected post-translational modifications.
- Host species (Mouse): Requires anti-mouse-IgG secondary reagents for indirect detection.
- Monoclonal format: Single-epitope binding provides high specificity and lot-to-lot reproducibility. Confirm epitope accessibility under your assay conditions.
- Isotype (IgG1): Matched secondary antibodies and isotype controls required. Compatible with standard Protein A/G purification workflows.
- Purification (Affinity chromatography): Enriches for specific immunoglobulin classes; reduces non-specific background vs. crude antisera.
Biological background
EGFP/EYFP (also referred to as GFP, Green Fluorescent Protein, enhanced Green Fluorescent Protein,) is a protein target studied in Bacillus cereus systems. Expression, subcellular localization, and post-translational modifications vary across cell types and disease states — factors critical to antibody-based detection design. Consult UniProt, NCBI Gene, and primary literature for current annotation of EGFP/EYFP biology in others.
Common research applications
- ELISA: Quantification of soluble target in biological fluids or culture supernatants. Ensure samples are within the linear detection range.
- WB: Confirms target molecular weight and distinguishes isoforms under denaturing conditions.
Notes for experimental interpretation
- Isotype controls: Use an isotype-matched (IgG1 from Mouse) control at equivalent concentration to assess non-specific background.
- Cross-reactivity: Confirm the clone does not cross-react with related family members under your assay conditions.
- Matrix effects: Sample matrix can affect performance; pilot dilution linearity and spike-recovery experiments are advised for quantitative studies.
- Species reactivity: Confirmed for Bacillus cereus. Extrapolation to untested species requires empirical validation.
This product is a monoclonal antibody produced from mouse hybridoma cells.
EGFP/EYFP is a protein target in Bacillus cereus biology. This monoclonal antibody raised in Mouse is designed to detect EGFP/EYFP in ELISA, WB applications, with IgG1 isotype.
Reported reactive against Bacillus cereus. Immunogen derived from Bacillus cereus. Cross-reactivity with other species should not be assumed without documented data or empirical testing.
Reported for ELISA, WB. Each format requires independent dilution optimization and appropriate controls. Unlisted applications require empirical testing.
This non-conjugated IgG1 antibody requires a compatible secondary. Select anti-mouse IgG secondary conjugated to your preferred reporter (HRP, AP, fluorophore, or biotin), matched to IgG1.
(1) Isotype control — IgG1 from Mouse at matching concentration; (2) Positive control — known EGFP/EYFP-expressing cell/tissue; (3) Negative control — knockdown/knockout sample for specificity; (4) Dilution linearity — verify proportional signal decrease in your matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
