| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | ETS-related transcription factor Elf-3; E74-like factor 3; Epithelial-restricted with serine box; Epithelium-restricted Ets protein ESX; Epithelium-specific Ets transcription factor 1; ESE-1; ELF3; ERT; ESX; JEN |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | Recombinant full length Human ELF3. |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage |
Product Overview
Research Use OnlyESE-1, a member of the Ets family of transcription factors, critically regulates epithelial cell differentiation and mediates vascular inflammation. ESE-1 is strongly expressed in vascular endothelium and smooth muscle cells where it is induced in response to inflammatory cytokines and lipopolysaccharides, interacts with NF-KappaB to induce nitric oxide synthase, and is induced during terminal differentiation of epidermal and primary keratinocytes. In addition, ESE-1 is upregulated upon differentiation of corneal epithelium and interacts with Sp1 and AP-1 proteins to induce squamous differenti
Validated Applications
| Western Blot (WB) | 500 - 1:2000 |
|---|---|
| Immunohistochemistry (IHC) | 50 - 1:200 |
| Immunofluorescence (IF/ICC) | See datasheet for recommended dilution |
Key Specifications
| Host Species | Rabbit |
|---|---|
| Species Reactivity | Human, Rat |
| Conjugate | Unconjugated |
| Purification | Affinity Purification |
| Formulation | Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 |
| Molecular Weight | ~ 41 kDa |
| Catalog No. | BS8164 |
| UniProt | P78545 |
| Alternative Names | ETS-related transcription factor Elf-3; E74-like factor 3; Epithelial-restricted with serine box; Epithelium-restricted Ets protein ESX; Epithelium-specific Ets transcription factor 1; ESE-1; ELF3; ERT; ESX; JEN |
Immunogen / Antigen
Recombinant full length Human ELF3.
Reactivity Notes
ELF3 polyclonal antibody detects endogenous levels of ELF3 protein.
Storage & Handling
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Safety
For Research Use Only (RUO). Not intended for diagnostic, therapeutic, or veterinary use. Handle according to standard laboratory biosafety guidelines. Avoid skin and eye contact. Dispose of waste in accordance with local regulations.
This antibody has been validated for: Western Blot, Immunohistochemistry, Immunofluorescence. For each application, follow the recommended dilutions on the product datasheet and optimize empirically for your sample type.
WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100
This antibody has been confirmed reactive against: Human, Rat. Cross-reactivity with other species may occur due to sequence homology but is not guaranteed without independent validation.
Recombinant full length Human ELF3.
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Avoid repeated freeze-thaw cycles. Centrifuge vials briefly before opening. Dilute immediately before use.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
