| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human TCEB2 was used as the immunogen for the ELOB antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
ELOB Antibody / Elongin B is a anti-ELOB Rabbit antibody Recombinant Rabbit Monoclonal clone 30E87 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.
Key elements and design rationale
- Target: ELOB
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30E87, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB
Biological background
ELOB antibody is widely applied in molecular biology, transcription, and ubiquitin proteasome system research. Through its participation in the elongin complex, ELOB regulates transcriptional control of genes involved in growth, survival, and stress responses. In ubiquitination, ELOB contributes to assembly of the von Hippel-Lindau tumor suppressor complex, which targets hypoxia-inducible factors for degradation. By detecting elongin-B, researchers can investigate how ELOB coordinates transcription and protein turnover.
Western blot assays detect ELOB protein in nuclear extracts, while immunohistochemistry maps expression in diverse tissues. Immunofluorescence reveals localization in nuclei and cytoplasmic ubiquitin ligase complexes. These applications enable comprehensive study of elongin-B across cellular pathways.
Altered ELOB function has been associated with cancer, where deregulated ubiquitination and transcription drive tumor progression. Mutations affecting the VHL-elongin-C-B complex contribute to renal cell carcinoma by stabilizing hypoxia-inducible factors. By applying ELOB antibody, scientists can explore its role in tumor suppression, protein degradation, and transcriptional regulation.
Beyond oncology, elongin-B plays roles in stress responses and cellular adaptation to hypoxia. Its broad functions reflect its integration of gene expression and protein stability mechanisms. This versatility makes ELOB antibody valuable for both basic and disease related research.
ELOB antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
