| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Storage |
- Endotoxin free (i.e., produced in mammalian cells and purified from serum free media)
- lycosylated for improved efficiency and stability
- Rapid cleavage (95% cleavage in 1hr)
- Highly specific with minimum non-specific cleavage (even at 20x concentration)
- Stable over broad temperature range
- His-tagged for convenient removal post reaction
| Specification | Value |
|---|---|
| Enzyme Type | Protease |
| Format | Enzyme Only |
| Storage Conditions | We recommend that the vial be briefly centrifuged prior to opening to bring the contents to the bottom. The stock solutions should be distributed into working aliquots and store at -20°C. Avoid multiple freeze/thaw cycles. Stable for 3 years from the date of shipping when stored and handled as per instructions. |
Enterokinase cleaves after the sequence D-D-D-D-K (DDDDK↓), which is typically placed between an affinity tag (His₆, GST, MBP) and the protein of interest. Cleavage leaves no additional residues on the N-terminus of the target protein.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
References
- Osakowicz C; Fletcher L; Caswell JL et al.. Protective and Anti-Inflammatory Effects of Protegrin-1 on Citrobacter rodentium Intestinal Infection in Mice. International journal of molecular sciences. 2021;22. DOI
- Laukkanen MO; Cammarota F; Esposito T et al.. Extracellular superoxide dismutase regulates the expression of small gtpase regulatory proteins GEFs, GAPs, and GDI. PloS one. 2015;10:e0121441. DOI
