| Field | Specification |
|---|---|
| Applications | |
| Storage | |
| Shelf life | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
The kit contains all the components needed to synthesize high-quality first-strand cDNA from total RNA or mRNA, and provides two primers for cDNA synthesis: Random Primers N6 and oligo (dT)18. The synthesized single-stranded cDNA product can be used directly in subsequent PCR or qPCR reactions.
First-strand cDNA is generated from 1 ng–5 µg of total RNA or 1 ng–500 ng of mRNA, and the cDNA produced can be amplified up to 10 kb. For qPCR the manual recommends no more than 1 µg of total RNA or 100 ng of mRNA per reaction. Reverse transcription is carried out in a 20 µL system at 25°C for 5 min, 42°C for 30 min and 85°C for 5 min, and the enzyme mix contains an RNase inhibitor.
Specifications
| Format | Reagent kit for first-strand cDNA synthesis |
|---|---|
| Starting material | Total RNA or mRNA |
| Sample input, total RNA | 1 ng–5 µg |
| Sample input, mRNA | 1 ng–500 ng |
| Product use | Full-length cDNA amplifiable up to 10 kb |
| Reaction volume | 20 µL |
| Reactions per kit | 100 rxn |
Kit Components
| Component | 100 rxn |
|---|---|
| RNase-Free ddH2O | 2 × 1 mL |
| 5× Buffer | 400 µL |
| EntiLink™ Enzyme Mix | 200 µL |
| Oligo (dT)18 (50 µM) | 100 µL |
| Random Primers N6 (50 µM) | 100 µL |
Applications
- First-strand cDNA synthesis from total RNA or mRNA
- Template preparation for PCR
- Template preparation for qPCR
- Reverse transcription of RNA templates with complex secondary structures
Key Features
- The EntiLink™ Reverse Transcriptase has dramatically improved thermal stability and can withstand reaction temperatures up to 50°C, making it suitable for reverse transcription of RNA templates with complex secondary structures.
- The EntiLink™ Reverse Transcriptase also has an increased affinity for templates and is suitable for reverse transcription of small amounts of templates and low-copy genes.
- The EntiLink™ Reverse Transcriptase also has an improved ability to synthesize full-length cDNAs, which can be amplified up to 10 kb in length.
Storage & Handling
All components are stored at -20°C. Synthesized cDNA is stored at -20°C for short-term storage and at -80°C for long-term storage.
Usage Notes
- Perform operations on ice.
- Avoid RNase contamination and use RNase-free tubes, filter-tip pipettes, gloves and masks.
- Store cDNA products at -20°C for short-term storage and at -80°C for long-term storage.
- For qPCR, use no more than 1 µg of total RNA or 100 ng of mRNA per reaction.
Safety & Regulatory
For research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.
Customization & Add-ons: Need this molecular biology reagent tailored to your workflow? We can help with bulk or custom pack sizes, alternative formats (spin column, magnetic bead or reagent-only kits; premixed or stand-alone reference dye), matched extraction, cDNA synthesis and qPCR workflows, and sourcing of related enzymes, ladders and consumables not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
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Peripheral nerves modulate the peri-implant osteogenesis under type 2 diabetes through exosomes derived from schwann cells via miR-15b-5p/Txnip signaling axis. JOURNAL OF NANOBIOTECHNOLOGY, 2025. 10.1186/s12951-025-03160-0
MiR-144-5p, an exosomal miRNA from bone marrow-derived macrophage in type 2 diabetes, impairs bone fracture healing via targeting Smad1. Journal of Nanobiotechnology, 2021. 10.1186/s12951-021-00964-8
PEX5R/Trip8b-HCN2 channel regulating neuroinflammation involved in perioperative neurocognitive disorders. Cell and Bioscience, 2022. 10.1186/s13578-022-00892-6
MiR-21 regulating PVT1/PTEN/IL-17 axis towards the treatment of infectious diabetic wound healing by modified GO-derived biomaterial in mouse models. Journal of Nanobiotechnology, 2022. 10.1186/s12951-022-01516-4
Decellularized extracellular matrix restores Fibronectin/Integrin β1 balance through extracellular vesicles to rejuvenate chondrocytes and alleviate osteoarthritis progression. Journal of Orthopaedic Translation, 2025. 10.1016/j.jot.2025.10.011
Multi-functional carboxymethyl chitin-based nanoparticles for modulation of tumor-associated macrophage polarity. Carbohydrate Polymers, 2021. Link
Characterization of Immune-Related Molecular Subtypes and a Prognostic Signature Correlating With the Response to Immunotherapy in Patients With Gastric Cancer. Frontiers in Immunology, 2022. Link
HIF1α lactylation enhances KIAA1199 transcription to promote angiogenesis and vasculogenic mimicry in prostate cancer. International Journal of Biological Macromolecules, 2022. Link
Exosomal miR-224-5p from Colorectal Cancer Cells Promotes Malignant Transformation of Human Normal Colon Epithelial Cells by Promoting Cell Proliferation through Downregulation of CMTM4. Oxidative Medicine and Cellular Longevity, 2022. Link
