| Field | Specification |
|---|---|
| Applications | |
| Storage | |
| Shelf life | |
| Catalog no. (Mfr.) | |
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Product Overview
EntiLink™ PCR Master Mix (Blue) is a ready-to-use, conventional PCR premix solution containing Taq DNA Polymerase, dNTP mixture, MgCl2 and an optimized buffer system. The reaction can be carried out by simply adding primers and templates, which greatly simplifies the experimental procedure. The product contains bromophenol blue dye, and the PCR product can be directly electrophoresed. This product contains excellent stabilizers and can be placed for 3 months at 4°C. The PCR product has a 3'-dA overhang and can be easily cloned into a T vector.
A 50 µL reaction is assembled from 25 µL of master mix, 1 µL of each 10 µM primer, template and ddH2O to 50 µL. Recommended template amounts are 50–200 ng of genomic DNA or 0.1–10 ng of plasmid DNA. Cycling is pre-denaturation at 94°C for 1–5 min, 35 cycles of 94°C for 30 sec, 50–60°C for 30 sec and 72°C for 30 sec/kb (60 sec/kb for cloning), and a final extension at 72°C for 10 min.
Specifications
| Format | Ready-to-use conventional PCR premix |
|---|---|
| Polymerase | Taq DNA Polymerase |
| Tracking dye | Bromophenol blue |
| Reaction volume | 50 µL |
| Template input, genomic DNA | 50–200 ng |
| Template input, plasmid DNA | 0.1–10 ng |
| Product ends | 3'-dA overhang for T vector cloning |
| Extension time | 30 sec/kb for identification; 60 sec/kb for cloning |
| Stability at 4°C | 3 months |
Applications
- Conventional PCR amplification
- Direct electrophoresis of PCR products
- Cloning of PCR products into T vectors
Storage & Handling
Stored at -20°C with a shelf life of two years for both catalogue sizes. The premix contains stabilizers and can be placed at 4°C for 3 months.
Usage Notes
- Set the annealing temperature 2–5°C below the primer Tm.
- Use an extension time of 30 sec/kb for identification and 60 sec/kb for cloning.
- Store at -20°C; the premix may be kept at 4°C for up to 3 months.
Safety & Regulatory
For research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.
Customization & Add-ons: Need this molecular biology reagent tailored to your workflow? We can help with bulk or custom pack sizes, alternative formats (spin column, magnetic bead or reagent-only kits; premixed or stand-alone reference dye), matched extraction, cDNA synthesis and qPCR workflows, and sourcing of related enzymes, ladders and consumables not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
Microbial profiling of artisanal goat milk and cheese: Insights into virulence traits, metabolic activity, and biofilm formation. Food Research International. 10.1016/j.foodres.2025.117434
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Remodeling tumor microenvironment using pH-sensitive biomimetic co-delivery of TRAIL/R848 liposomes against colorectal cancer. Oncology Research, 2024. 10.32604/or.2024.045564
