| Field | Specification |
|---|---|
| Applications | |
| Storage | |
| Shelf life | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
EntiLink™ PCR Master Mix (Red) is a ready-to-use conventional PCR master mix solution containing Taq DNA Polymerase, dNTP mix, MgCl2, and optimized buffer system. The amplification can be performed by adding primers and templates during the reaction, which greatly simplifies the operation steps of the experiment. The product contains cresol red, and the PCR product can be directly electrophoresed. This product contains excellent stabilizer and can be stored for 3 months at 4°C. PCR products have 3'-dA overhangs for easy cloning into T-vectors.
A 50 µL reaction is assembled from 25 µL of master mix, 1 µL of each 10 µM primer, a moderate amount of template and ddH2O to 50 µL. Recommended template amounts are 50–200 ng of genomic DNA or 0.1–10 ng of plasmid DNA. Cycling is pre-denaturation at 94°C for 1–5 min, 35 cycles of 94°C for 30 sec, 50–60°C for 30 sec and 72°C for 30 sec/kb (60 sec/kb for gene cloning), and a final extension at 72°C for 10 min.
Specifications
| Format | Ready-to-use conventional PCR master mix |
|---|---|
| Polymerase | Taq DNA Polymerase |
| Tracking dye | Cresol red |
| Reaction volume | 50 µL |
| Template input, genomic DNA | 50–200 ng |
| Template input, plasmid DNA | 0.1–10 ng |
| Product ends | 3'-dA overhangs for T-vector cloning |
| Extension time | 30 sec/kb; 60 sec/kb for gene cloning |
| Stability at 4°C | 3 months |
Applications
- Conventional PCR amplification
- Direct electrophoresis of PCR products
- Cloning of PCR products into T-vectors
Storage & Handling
Stored at -20°C with a shelf life of two years for both catalogue sizes. The product contains stabilizer and can be stored for 3 months at 4°C.
Usage Notes
- Be sure to mix the master mix thoroughly before use.
- Set the annealing temperature 2–5°C below the theoretical primer Tm.
- Use an extension time of 30 sec/kb, or 60 sec/kb for gene cloning.
Safety & Regulatory
For research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.
Customization & Add-ons: Need this molecular biology reagent tailored to your workflow? We can help with bulk or custom pack sizes, alternative formats (spin column, magnetic bead or reagent-only kits; premixed or stand-alone reference dye), matched extraction, cDNA synthesis and qPCR workflows, and sourcing of related enzymes, ladders and consumables not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
