| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human ENTPD5 recombinant protein (Position: D95-H428) was used as the immunogen for the ENTPD5 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
ENTPD5 Antibody / Ectonucleoside triphosphate diphosphohydrolase 5 is a anti-ENTPD5 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: ER.
Key elements and design rationale
- Target: ENTPD5
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, FACS, ELISA
Biological background
Functionally, ENTPD5 antibody identifies a 428-amino-acid enzyme that hydrolyzes UDP and GDP to UMP and GMP, coupling its activity to the UDP-glucose pyrophosphorylase reaction that regenerates UDP-glucose. This cycle supports proper glycoprotein processing by maintaining a continuous supply of glycosylation precursors within the ER. ENTPD5 activity enhances protein folding capacity and assists in the maturation of membrane receptors and secretory proteins.
The ENTPD5 gene is located on chromosome 14q24.1 and is expressed in metabolically active tissues such as liver, pancreas, and muscle. Its expression is transcriptionally upregulated by AKT signaling and MYC oncogene activation, linking metabolic reprogramming to cancer cell growth. ENTPD5 contributes to the Warburg effect by coupling glycosylation demand to glycolytic flux in proliferating cells.
Pathologically, overexpression of ENTPD5 promotes tumor progression by increasing glycoprotein folding and surface receptor stability, enhancing proliferative signaling. Conversely, loss of ENTPD5 impairs growth factor receptor maturation and induces ER stress. Elevated ENTPD5 expression has been observed in prostate, colon, and liver cancers. Research using ENTPD5 antibody supports studies in cancer metabolism, glycoprotein folding, and ER homeostasis.
ENTPD5 antibody is validated for western blotting, immunohistochemistry, and ELISA to detect ER-localized glycoprotein processing enzymes.
Structurally, Ectonucleoside triphosphate diphosphohydrolase 5 contains an apyrase conserved region (ACR) and calcium-binding motifs essential for nucleotide hydrolysis. It forms dimers in the ER lumen and associates with chaperones such as calnexin and calreticulin. This antibody enables exploration of ENTPD5's role in glycoprotein biosynthesis, ER stress adaptation, and cancer metabolism.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
