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Product Overview
EnTurbo™ Probe qPCR SuperMix is an optimized 2× real-time PCR master mix containing HotStarTaq DNA Polymerase, dNTP and Mg2+. In addition, the balanced K+ and NH4+ ion ratios in the buffer promote specific primer annealing. To ensure a highly sensitive and specific PCR reaction, the reaction can be initiated by simply adding the primer and cDNA template to the ready-to-use PCR master mix. The unique PCR buffer ensures sensitive qPCR on all real-time PCR instruments without optimization.
The SuperMix is supplied with a stand-alone 50× ROX Dye and RNase-Free ddH2O in a 20 µL × 500 rxn pack, and the HotStarTaq DNA Polymerase suppresses non-specific amplification. Input per 20 µL reaction is 10 pg–1 ng of cDNA or 100 pg–1 ng of plasmid or genomic DNA. Reactions use 10 µL of SuperMix in a 20 µL system or 5 µL in a 10 µL system, and the amount of ROX dye depends on the instrument. Cycling is 95°C for 30 sec, then 35–40 cycles of 95°C for 5 sec and 60°C for 30 sec (two-step), or of 95°C for 5 sec, 50–60°C for 30 sec and 72°C for 30 sec (three-step).
Specifications
| Concentration | 2× real-time PCR master mix |
|---|---|
| Polymerase | HotStarTaq DNA Polymerase (hot start) |
| Detection chemistry | Probe-based qPCR |
| ROX | Stand-alone 50× ROX Dye, 1 mL |
| Probe and primers | Not supplied; labelled probe and primers added by the user |
| Template input, cDNA | 10 pg–1 ng per 20 µL reaction |
| Template input, plasmid or genomic DNA | 100 pg–1 ng per 20 µL reaction |
| Reaction volume | 20 µL or 10 µL |
Kit Components
| Component | 20 µL × 500 rxn |
|---|---|
| EnTurbo™ Probe qPCR SuperMix | 4 × 1.25 mL |
| 50× ROX Dye | 1 mL |
| RNase-Free ddH2O | 4 × 1.25 mL |
Applications
- cDNA gene expression analysis
- Absolute quantification of plasmids
- Absolute quantification of genomic DNA
- Quantification of sequencing libraries
Key Features
- Quickly get results, saving up to 50% of the time
- Optimized ready-to-use master mix for rapid PCR reactions
- Accurate detection of various starting amounts of templates, stable amplification, quantitative results with high repeatability
- Balanced K+ and NH4+ ion ratios, as well as stand-alone ROX Reference Dye packaging for all real-time PCR instruments
Storage & Handling
Stored at -20°C for long-term storage with a shelf life of one year; the SuperMix can also be kept at 2–8°C in the dark. Shipping: Transport on ice or dry ice.
Usage Notes
- Wear a lab coat and disposable gloves when performing the experiment.
- Use gradient dilution for high-abundance genes to avoid low Ct values.
- Mix by inversion before use.
- Transport on ice or dry ice.
- Use at least three biological replicates.
Safety & Regulatory
For research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.
Customization & Add-ons: Need this molecular biology reagent tailored to your workflow? We can help with bulk or custom pack sizes, alternative formats (spin column, magnetic bead or reagent-only kits; premixed or stand-alone reference dye), matched extraction, cDNA synthesis and qPCR workflows, and sourcing of related enzymes, ladders and consumables not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
