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Product Overview
EnTurbo™ SYBR Color qPCR SuperMix is an optimized 2× real-time PCR master mix containing HotStarTaq DNA Polymerase, SYBR Green I fluorochrome dye, dNTP and Mg2+ and High ROX. In addition, the balanced K+ and NH4+ ion ratios in the buffer promote specific primer annealing. To ensure a highly sensitive and specific PCR reaction, the reaction can be initiated by simply adding the primer and cDNA template to the ready-to-use PCR master mix. The unique PCR buffer ensures sensitive qPCR.
In addition, the product visualizes the pipetting process through the colour change reaction after the template is added, which greatly improves pipetting efficiency and avoids pipetting errors. The 10 × Dilution Buffer provides the blue-to-green colour tracking. Per 20 µL reaction the manual specifies cDNA from 10 pg–1 ng of total RNA; for plasmid or genomic DNA it specifies 100 pg–1 ng, or 10¹–10⁷ copies. A 20 µL reaction contains 10 µL of master mix, 0.4 µL of each 10 µM primer, template and ddH2O, and is run at 95°C for 30 sec, then 35–40 cycles of 95°C for 5 sec and 60°C for 30 sec (two-step; the three-step program adds a 72°C extension for 30 sec), followed by melt curve analysis.
Specifications
| Concentration | 2× real-time PCR master mix |
|---|---|
| Polymerase | HotStarTaq DNA Polymerase (hot start) |
| Detection chemistry | SYBR Green I fluorochrome dye |
| ROX | High ROX premixed |
| Colour tracking | Blue-to-green colour change from the 10 × Dilution Buffer |
| Template input, cDNA | Derived from 10 pg–1 ng of total RNA per 20 µL reaction |
| Template input, plasmid or genomic DNA | 100 pg–1 ng, or 10¹–10⁷ copies per 20 µL reaction |
| Reaction volume | 20 µL |
Kit Components
| Component | 20 µL × 500 rxn |
|---|---|
| 2 × SYBR Color qPCR Mix (High ROX) | 4 × 1.25 mL |
| 10 × Dilution Buffer | 1 mL |
| ddH2O | 4 × 1.25 mL |
Applications
- Gene expression analysis of cDNA
- Absolute quantification of plasmids
- Absolute quantification of genomic DNA
- Quantification of sequencing libraries
Key Features
- Quickly get results, saving up to 50% of the time
- Optimized ready-to-use master mix for rapid PCR reactions
- Accurate detection of various starting amounts of templates, stable amplification, quantitative results with high repeatability
- Balanced K+ and NH4+ ion ratios, ensure high sensitivity and high specificity.
- Reduces pipetting errors by tracking the pipetting process through the color change reaction between dyes
Storage & Handling
Stored at -20°C with a shelf life of one year. Avoid strong light during storage and reaction formulation. Shipping: Ice bag, dry ice transport.
Usage Notes
- Avoid strong light during storage and reaction formulation.
- Transport on an ice bag or dry ice.
- Template above 100 ng of cDNA used undiluted may cause low Ct values, which affects the accuracy of the result.
- The Dilution Buffer produces a blue-to-green colour change that tracks template addition.
Safety & Regulatory
For research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.
Customization & Add-ons: Need this molecular biology reagent tailored to your workflow? We can help with bulk or custom pack sizes, alternative formats (spin column, magnetic bead or reagent-only kits; premixed or stand-alone reference dye), matched extraction, cDNA synthesis and qPCR workflows, and sourcing of related enzymes, ladders and consumables not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
