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Product Overview
EnTurbo™ SYBR Color qPCR SuperMix is an optimized 2× real-time PCR master mix containing HotStarTaq DNA Polymerase, SYBR Green® fluorescent dye, dNTP and Mg2+, Low ROX. In addition, the balanced K+ and NH4+ ion ratios in the buffer promote specific primer annealing. To ensure a highly sensitive and specific PCR reaction, the reaction can be initiated by simply adding the primer and cDNA template to the ready-to-use PCR master mix. This simplifies the workflow and reduces the risk of contamination. The unique PCR buffer ensures sensitive qPCR.
In addition, the product realizes the visualization of the sample addition process through the discoloration reaction after the template is added, which greatly improves the sample addition efficiency and avoids sample addition errors. A blue-to-green colour change indicates that template has been added. Per 20 µL reaction the manual specifies 10 pg–1 ng of cDNA, 10¹–10⁷ copies of plasmid DNA or 100 pg–1 ng of genomic DNA. A 20 µL reaction contains 10 µL of master mix, 0.4 µL of each primer, template and ddH2O, and is run at 95°C for 30 sec, then 35–40 cycles of 95°C for 5 sec and 60°C for 30 sec, followed by melting curve analysis.
Specifications
| Concentration | 2× real-time PCR master mix |
|---|---|
| Polymerase | HotStarTaq DNA Polymerase (hot start) |
| Detection chemistry | SYBR Green dye |
| ROX | Low ROX premixed |
| Colour tracking | Blue-to-green colour change indicates template addition |
| Template input, cDNA | 10 pg–1 ng per 20 µL reaction |
| Template input, plasmid DNA | 10¹–10⁷ copies per 20 µL reaction |
| Template input, genomic DNA | 100 pg–1 ng per 20 µL reaction |
| Reaction volume | 20 µL |
Kit Components
| Component | 20 µL × 500 rxn |
|---|---|
| 2 × SYBR Color qPCR Mix (Low ROX) | 4 × 1.25 mL |
| 10 × Dilution Buffer | 1 mL |
| ddH2O | 4 × 1.25 mL |
Applications
- cDNA gene expression analysis
- Plasmid quantification
- Quantification of genomic DNA
- Quantification of sequencing libraries
Key Features
- Quickly get results, saving up to 50% of the time
- Optimized ready-to-use premix for rapid PCR reactions
- Accurate detection of various starting amounts of templates, stable amplification, quantitative results with high repeatability
- Balanced K+ and NH4+ ion ratios, ensure high sensitivity and high specificity
- Track the pipetting process and reduce pipetting errors through the color change reaction between dyes
Storage & Handling
Stored at -20°C with a shelf life of one year. Avoid strong light during storage and reaction setup.
Usage Notes
- Avoid strong light during storage and reaction setup.
- Wear a lab coat and gloves when performing the experiment.
- Use at least three biological replicates.
- Increase the pre-denaturation step to 2 min for high-GC or complex templates.
Safety & Regulatory
For research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.
Customization & Add-ons: Need this molecular biology reagent tailored to your workflow? We can help with bulk or custom pack sizes, alternative formats (spin column, magnetic bead or reagent-only kits; premixed or stand-alone reference dye), matched extraction, cDNA synthesis and qPCR workflows, and sourcing of related enzymes, ladders and consumables not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
