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Product Overview
EnTurbo™ SYBR Color qPCR SuperMix is an optimized 2× real-time PCR master mix containing HotStarTaq DNA Polymerase, SYBR Green® fluorescent dye, dNTP and Mg2+. In addition, the balanced K+ and NH4+ ion ratios in the buffer promote specific primer annealing. To ensure a highly sensitive and specific PCR reaction, the reaction can be initiated by simply adding the primer and cDNA template to the ready-to-use PCR master mix. This simplifies the workflow and reduces the risk of contamination. The unique PCR buffer ensures sensitive qPCR.
In addition, the product realizes the visualization of the sample addition process through the discoloration reaction after the template is added, which greatly improves the sample addition efficiency. This version contains no ROX. The 10 × Dilution Buffer enables visual pipetting tracking through a blue-to-green colour change. The HotStarTaq DNA Polymerase is activated at 95°C for 30 sec, and per 20 µL reaction the manual specifies 10 pg–1 ng of cDNA, 100 pg–1 ng of genomic DNA or 10¹–10⁷ copies of plasmid DNA.
Specifications
| Concentration | 2× real-time PCR master mix |
|---|---|
| Polymerase | HotStarTaq DNA Polymerase (hot start, activated at 95°C for 30 sec) |
| Detection chemistry | SYBR Green® fluorescent dye |
| ROX | Without ROX |
| Colour tracking | Blue-to-green colour change from the 10 × Dilution Buffer |
| Template input, cDNA | 10 pg–1 ng per 20 µL reaction |
| Template input, genomic DNA | 100 pg–1 ng per 20 µL reaction |
| Template input, plasmid DNA | 10¹–10⁷ copies per 20 µL reaction |
| Reaction volume | 20 µL |
Kit Components
| Component | 20 µL × 500 rxn |
|---|---|
| 2 × SYBR Color qPCR Mix (Without ROX) | 4 × 1.25 mL |
| 10 × Dilution Buffer | 1 mL |
| ddH2O | 4 × 1.25 mL |
Applications
- cDNA gene expression analysis
- Absolute quantification of plasmids
- Absolute quantification of genomic DNA
- Quantification of sequencing libraries
Key Features
- Quickly get results, saving up to 50% of the time
- Optimized ready-to-use premix for rapid PCR reactions
- Accurate detection of various starting amounts of templates, stable amplification, quantitative results with high repeatability
- Balanced K+ and NH4+ ion ratios, ensure high sensitivity and high specificity
- Track the pipetting process and reduce pipetting errors through the color change reaction between dyes
Storage & Handling
Stored at -20°C in the dark with a shelf life of one year. Avoid strong light during storage. Shipping: Ice bag, dry ice transport.
Usage Notes
- Avoid strong light during storage.
- Wear a lab coat and disposable gloves when performing the experiment.
- Mix by inversion before use.
- Use gradient dilution for high-abundance genes.
Safety & Regulatory
For research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.
Customization & Add-ons: Need this molecular biology reagent tailored to your workflow? We can help with bulk or custom pack sizes, alternative formats (spin column, magnetic bead or reagent-only kits; premixed or stand-alone reference dye), matched extraction, cDNA synthesis and qPCR workflows, and sourcing of related enzymes, ladders and consumables not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
