| Field | Specification |
|---|---|
| Applications | |
| Storage | |
| Shelf life | |
| Catalog no. (Mfr.) | |
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Product Overview
EnTurbo™ SYBR Green PCR SuperMix is an optimized 2× real-time PCR master mix containing HotStarTaq DNA Polymerase, SYBR Green® fluorescent dye, dNTP and Mg2+. In addition, the balanced K+ and NH4+ ion ratios in the buffer promote specific primer annealing. To ensure a highly sensitive and specific PCR reaction, the reaction can be initiated by simply adding the primer and cDNA template to the ready-to-use PCR master mix. The unique PCR buffer ensures sensitive qPCR on all real-time PCR instruments without optimization.
The documented pack is 20 µL × 500 rxn, which corresponds to the 5 mL option and contains 4 × 1.25 mL of SuperMix with a stand-alone 50× ROX Dye and RNase-Free ddH2O; it is used for gene expression analysis and absolute quantitative analysis. Input per 20 µL reaction is 10 pg–1 ng of cDNA; for plasmid or genomic DNA the input is 100 pg–1 ng, or 10–10⁷ copies. The manual reports a Ct value range of 5–35, single-digit copy detection and run time reduced by 50%.
Specifications
| Concentration | 2× real-time PCR master mix |
|---|---|
| Polymerase | HotStarTaq DNA Polymerase (hot start) |
| Detection chemistry | SYBR Green® fluorescent dye |
| ROX | Stand-alone 50× ROX Dye supplied separately |
| Template input, cDNA | 10 pg–1 ng per reaction |
| Template input, plasmid or genomic DNA | 100 pg–1 ng, or 10–10⁷ copies per 20 µL reaction |
| Reaction volume | 20 µL |
| Ct value range | 5–35 |
| Sensitivity | Single-digit copy detection |
Kit Components
| Component | 20 µL × 500 rxn |
|---|---|
| EnTurbo™ SYBR Green PCR SuperMix | 4 × 1.25 mL |
| 50× ROX Dye | 1 mL |
| RNase-Free ddH2O | 4 × 1.25 mL |
Applications
- Gene expression analysis
- Absolute quantitative analysis
Key Features
- Quickly get results, saving up to 50% of the time
- Optimized ready-to-use master mix for rapid PCR reactions
- Accurate detection of various starting amounts of templates, stable amplification, quantitative results with high repeatability
- Balanced K+ and NH4+ ion ratios, as well as stand-alone ROX Reference Dye packaging for all real-time PCR instruments
Storage & Handling
Stored at -20°C with a shelf life of one year. Avoid strong light during storage.
Usage Notes
- Avoid strong light during storage.
- Wear a lab coat and disposable gloves when performing the experiment.
- Mix by inversion before use.
Safety & Regulatory
For research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.
Customization & Add-ons: Need this molecular biology reagent tailored to your workflow? We can help with bulk or custom pack sizes, alternative formats (spin column, magnetic bead or reagent-only kits; premixed or stand-alone reference dye), matched extraction, cDNA synthesis and qPCR workflows, and sourcing of related enzymes, ladders and consumables not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
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