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Product Overview
EnTurbo™ SYBR Green PCR SuperMix is an optimized 2× real-time PCR master mix containing HotStarTaq DNA Polymerase, SYBR Green® fluorescent dye, dNTP and Mg2+. In addition, the balanced K+ and NH4+ ion ratios in the buffer promote specific primer annealing. To ensure a highly sensitive and specific PCR reaction, the reaction can be initiated by simply adding the primer and cDNA template to the ready-to-use PCR master mix. The unique PCR buffer ensures sensitive qPCR.
In this version Low ROX is premixed in the master mix. Input per reaction is 10 pg–1 ng of cDNA; for genomic DNA or plasmid the input is 100 pg–1 ng, or 10–10⁷ copies. Reactions are run in 10 µL, 20 µL or 50 µL with primers at 0.2 µM final concentration, and the manual reports a Ct value range of 5–35 with single-digit copy detection.
Specifications
| Concentration | 2× real-time PCR master mix |
|---|---|
| Polymerase | HotStarTaq DNA Polymerase (hot start) |
| Detection chemistry | SYBR Green® fluorescent dye |
| ROX | Low ROX premixed |
| Template input, cDNA | 10 pg–1 ng per reaction |
| Template input, genomic DNA or plasmid | 100 pg–1 ng, or 10–10⁷ copies |
| Reaction volume | 10 µL, 20 µL or 50 µL |
| Ct value range | 5–35 |
| Sensitivity | Single-digit copy detection |
Kit Components
| Component | 20 µL × 500 rxn |
|---|---|
| 2× SYBR Green PCR Master Mix (Low ROX Premixed) | 4 × 1.25 mL |
| RNase-Free ddH2O | 4 × 1.25 mL |
Applications
- Gene expression analysis
- Absolute quantification of plasmids
- Absolute quantification of genomic DNA
- Quantification of sequencing libraries
Key Features
- Quickly get results, saving up to 50% of the time
- Optimized ready-to-use master mix for rapid PCR reactions
- Accurate detection of various starting amounts of templates, stable amplification, quantitative results with high repeatability
Storage & Handling
Stored at -20°C with a shelf life of one year, protected from strong light.
Usage Notes
- Wear a lab coat and disposable gloves when performing the experiment.
- Mix by inversion before use.
- Avoid strong light.
- Use gradient dilution for high-abundance cDNA templates.
- Use at least three biological replicates and confirm amplification and melting curves.
Safety & Regulatory
For research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.
Customization & Add-ons: Need this molecular biology reagent tailored to your workflow? We can help with bulk or custom pack sizes, alternative formats (spin column, magnetic bead or reagent-only kits; premixed or stand-alone reference dye), matched extraction, cDNA synthesis and qPCR workflows, and sourcing of related enzymes, ladders and consumables not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
Chronic changes developing in the hydronephrotic and contralateral kidneys during unilateral ureteral obstruction in rats. Molecular Biology Reports, 2025. 10.1007/s11033-025-10486-3
Morphological and Molecular Changes in Renal Tissue Following Experimental Unilateral Ureteral Obstruction in Rat Kidneys. Kafkas Universitesi Veteriner Fakultesi Dergisi, 2024. 10.9775/kvfd.2024.32776
