| Field | Specification |
|---|---|
| Assay Time | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Serum, plasma etc |
| Shipping | |
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| Storage |
Overview
For quantitative determination of cholesterol and evaluation of drug effects on cholesterol metabolism. The assay uses OD340nm for signal readout. Compatible sample input includes Serum, plasma etc. Typical stated assay timing is 40 min.
Key elements and design rationale
- Readout format: OD340nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Serum, plasma etc, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 5 mg/dL for interpreting low-signal samples.
- Feature emphasis: Sensitive and accurate. The detection limit of 5 mg/dL, linearity up to 300 mg/dL cholesterol in a 96-well plate assay.
Additional feature notes highlight Convenient. Room temperature assay. No 37°C heater is needed; High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Available format information for this listing includes 100 Tests.
Biological background
This product is centered on measurement of cholesterol within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
CHOLESTEROL is a sterol and lipid present in the cell membranes, and is transported in the bloodstream of all animals. It is used to form cell membranes and hormones and plays important roles in cell signaling processes. Elevated levels (hypercholesterolemia) have been associated with cardiovascular diseases such as atherosclerosis; whereas, low levels (hypocholesterolemia) may be linked to depression, cancer and cerebral haemorrhage. Simple, direct and automation-ready procedures for measuring cholesterol are very desirable. BioAssay Systems EnzyChrom™ Cholesterol Assay is based on cholesterol esterase hydrolysis of cholesterol esters to form free cholesterol and cholesterol dehydrogenase catalyzed conversion of cholesterol to cholest-4-ene-3-one, in which NAD is reduced to NADH. The optical density of the formed NADH at 340 nm is directly proportionate to the cholesterol concentration in the sample.
Detection method
Colorimetric (OD 340 nm).
Detection limit and analytical sensitivity
Reported detection limit: 5 mg/dL.
Procedures and timing
Stated procedure or timing information: 40 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.
Common research applications
- Quantify cholesterol in serum, plasma by OD340 nm readout.
- Compare treatment or phenotype groups using matched serum, plasma handling.
- Monitor time-course or pre/post changes in serum, plasma across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
I would like to know whether egg yolk can be used for such a kit. If this doesn’t work, can you suggest us another protocol, please?
Egg yolk cholesterol extraction protocol:
1. Prepare extraction buffer with 5 vol isopropanol, 2 vol water, 2 vol Triton X-100.
In a 1.5mL Eppendorf tube, mix 50µL yolk with 450 µL of the extraction buffer. Vortex for at least 30 sec.
3. Centrifuge 4 min at 14,000rpm on a table centrifuge.
4. Remove supernatant and perform cholesterol assay.
What is the principle of the ECCH-100 cholesterol assay?
Cholesterol esterase cleaves esters into cholesterol, which is oxidized to form NADH in the presence of cholesterol dehydrogenase. The produced NADH is measured colorimetrically.
Will the assay detect the cholesterol in the lipoproteins, or only free-cholesterol in the serum?
The assay will detect total serum cholesterol, i.e. protein-bound serum (VLDL/LDL/HDL) and free cholesterol.<
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
Associations of Circulating Irisin with FNDC5 Expression in Fat and Muscle in Type 1 and Type 2 Diabetic Mice
Jiang, S.,et al. (2021). Associations of Circulating Irisin with FNDC5 Expression in Fat and Muscle in Type 1 and Type 2 Diabetic Mice. Biomolecules. 11(2): 322. Assay: Cholesterol in mouse plasma.
Variation of body condition and plasma energy substrates with life stage, sex, and season in wild-sampled nurse sharks Ginglymostoma cirratum
Moorhead, S. G., et al. (2020). Variation of body condition and plasma energy substrates with life stage, sex, and season in wild-sampled nurse sharks Ginglymostoma cirratum . Journal of Fish Biology. jfb: 14612. Assay: Cholesterol in shark plasma.
Serum metabolites and body condition score associated with metritis, endometritis, ketosis, and mastitis in Holstein cows
Torres, E., et al. (2020). Serum metabolites and body condition score associated with metritis, endometritis, ketosis, and mastitis in Holstein cows. Pesquisa Agropecuaria Brasileira. 55: e01308. Assay: Cholesterol in cow serum.
Physiological status of silver carp (Hypophthalmichthys molitrix) in the Illinois River: An assessment of fish at the leading edge of the invasion front
Jeffrey, J. D., et al. (2019). Physiological status of silver carp (Hypophthalmichthys molitrix) in the Illinois River: An assessment of fish at the leading edge of the invasion front. Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics. 32, 100614. Assay: Cholesterol in silver carp plasma.
Capsaicin suppresses liver fat accumulation in high-fat diet-induced NAFLD mice
Shin, M. K., et al. (2020). Capsaicin suppresses liver fat accumulation in high-fat diet-induced NAFLD mice. Animal Cells and Systems. 24(4): 214-219. Assay: Cholesterol in mouse plasma.
Cholesterol deficiency carriers have lowered serum cholesterol and perform well at an elite cattle show
Basiel, B. L., et al. (2020). Cholesterol deficiency carriers have lowered serum cholesterol and perform well at an elite cattle show. JDS Communications. 1(1): 6-9. Assay: Cholesterol in cattle serum.
Evaluation of changes in body composition and proinflammatory marker levels in ovariectomized rats with induced hyperthyroidism
Yoon, J. A., et al. (2020). Evaluation of changes in body composition and proinflammatory marker levels in ovariectomized rats with induced hyperthyroidism. All Life. 13(1): 99-107. Assay: Cholesterol in rat serum.
Potentiated in vitro probiotic activities of lactobacillus fermentum lfqi6 biofilm biomass versus planktonic culture
Berkes, E., et al. (2020). Potentiated in vitro probiotic activities of lactobacillus fermentum lfqi6 biofilm biomass versus planktonic culture. Probiotics and Antimicrobial Proteins. 12(3): 1097-1114. Assay: Cholesterol in Lactobacillus fermentum LfQi6 culture.
The effect of Mutagens agent Ethidium bromide on some therapeutic properties for local Iraqi isolate Saccharomyces boulardii
Al-Ziadi, R. E (2017). The effect of Mutagens agent Ethidium bromide on some therapeutic properties for local Iraqi isolate Saccharomyces boulardii. Scientific Journal of Medical Research 1.1: 13-18. Assay: Cholesterol in Saccharomyces boulardii (Yeast) culture medium.
A high-cholesterol diet increases 27-hydroxycholesterol and modifies estrogen receptor expression and neurodegeneration in rabbit hippocampus
Brooks, Sylwia W., Ava C. Dykes, and Bernard G. Schreurs (2017). A high-cholesterol diet increases 27-hydroxycholesterol and modifies estrogen receptor expression and neurodegeneration in rabbit hippocampus. Journal of Alzheimer’s Disease 56.1 (2017): 185-196. Assay: Cholesterol in rabbits serum.
Effects of mangosteen peel (Garcinia mangostana) and ginger rhizome (Curcuma xanthorrhiza) on the performance and cholesterol levels of heat-stressed broiler chickens
Hidanah, Sri, et al (2017). Effects of mangosteen peel (Garcinia mangostana) and ginger rhizome (Curcuma xanthorrhiza) on the performance and cholesterol levels of heat-stressed broiler chickens. Pakistan Journal of Nutrition 16.1: 28-32. Assay: Cholesterol in broiler chicks tissues.
Marine microalgae as dietary supplements in the culture of juvenile Chinese horseshoe crabs, Tachypleus tridentatus (Xiphosura)
Kwan, Billy KY, et al (2017). Marine microalgae as dietary supplements in the culture of juvenile Chinese horseshoe crabs, Tachypleus tridentatus (Xiphosura). Aquaculture research 48.7: 3910-3924. Assay: Cholesterol in horseshoe crabs plasma.
Effect of quality of colostrum on health, growth and immunoglobulin G concentration in Holstein calves in a hot environment
Mellado, Miguel, et al (2017). Effect of quality of colostrum on health, growth and immunoglobulin G concentration in Holstein calves in a hot environment. Animal Science Journal 88.9: 1327-1336. Assay: Cholesterol in calves serum.
Beta-carotene supplementation positively affects selected blood metabolites across time around the onset of puberty in goats
Meza-Herrera, Cesar A., et al (2017). Beta-carotene supplementation positively affects selected blood metabolites across time around the onset of puberty in goats. Czech Journal of Animal Science 62.1 (2017): 22-31. Assay: Cholesterol in goats serum.
Microbiome and metabolome modifying effects of several cardiovascular disease interventions in apo-E-/- mice
Ryan, Paul M., et al (2017). Microbiome and metabolome modifying effects of several cardiovascular disease interventions in apo-E-/- mice. Microbiome 5.1: 30. Assay: Cholesterol in mice serum.
Recombinant incretin-secreting microbe improves metabolic dysfunction in high-fat diet fed rodents
Ryan, Paul M., et al (2017). Recombinant incretin-secreting microbe improves metabolic dysfunction in high-fat diet fed rodents. Scientific reports 7.1: 13523. Assay: Cholesterol in rodent serum.
Exopolysaccharide-producing bacteria, and uses thereof for protecting heart health
Stanton, Catherine, et al (2017). Exopolysaccharide-producing bacteria, and uses thereof for protecting heart health. U.S. Patent No. 9,808,493. Assay: Cholesterol in mice serum.
Physiological and behavioral basis for the successful adaptation of goats to severe water restriction under hot environmental conditions
Kaliber, M., N. Koluman, and N. Silanikove (2016). Physiological and behavioral basis for the successful adaptation of goats to severe water restriction under hot environmental conditions. animal 10.1: 82-88. Assay: Cholesterol in goats plasma.
Watershed-scale land use activities influence the physiological condition of stream fish
King, Gregory D., et al (2016). Watershed-scale land use activities influence the physiological condition of stream fish. Physiological and Biochemical Zoology 89.1: 10-25. Assay: Cholesterol in fish plasma.
Physiological consequences of hybridization: early generation backcrossing decreases performance in invasive bigheaded carps
Liss, Stephanie A., et al (2016). Physiological consequences of hybridization: early generation backcrossing decreases performance in invasive bigheaded carps. Journal of Freshwater Ecology 31.4: 543-554. Assay: Cholesterol in fish plasma.
Spatial and temporal influences on the physiological condition of invasive silver carp
Liss, S. et al (2013). Spatial and temporal influences on the physiological condition of invasive silver carp. Conserv Physiol. 1 (1): cot017 Assay: Cholesterol in fish (silver carp) plasma.
Modulation of skeletal muscle performance and SERCA by exercise and adiponectin gene therapy in insulin-resistant rat
Safwat Y, et al (2013). Modulation of skeletal muscle performance and SERCA by exercise and adiponectin gene therapy in insulin-resistant rat. DNA Cell Biol. 32(7):378-85. Assay: Cholesterol in rat plasma.
Modulation of lipid metabolism by mixtures of protamine and chitooligosaccharide through pancreatic lipase inhibitory activity in a rat model
Kang, NH et al (2012). Modulation of lipid metabolism by mixtures of protamine and chitooligosaccharide through pancreatic lipase inhibitory activity in a rat model. Lab Anim Res ;8(1):31-8. Assay: Cholesterol in rat feces.
Risk of cardiovascular disease is suppressed by dietary supplementation with protamine and chitooligosaccharide in Sprague-Dawley rats
Kang, NH et al (2012). Risk of cardiovascular disease is suppressed by dietary supplementation with protamine and chitooligosaccharide in Sprague-Dawley rats. Mol Med Rep. ;7(1):127-33. Assay: Cholesterol in rat feces.
Effect of dietary energy intake and somatotropin administration after weaning on growth rate and semen characteristics of Granadina goat bucks
Mellado, M et al (2012). Effect of dietary energy intake and somatotropin administration after weaning on growth rate and semen characteristics of Granadina goat bucks. Turk. J. Vet. Anim. Sci. 36(4): 338-345. Assay: Cholesterol in goat buck sersum.
Regular Exercise or Changing Diet Does Not Influence Aortic Valve Disease Progression in LDLR Deficient Mice
Schlotter F, Matsumoto Y, Mangner N, Schuler G, Linke A, et al. (2012). Regular Exercise or Changing Diet Does Not Influence Aortic Valve Disease Progression in LDLR Deficient Mice. PLoS ONE 7(5): e37298. Assay: Cholesterol in mice serum.
alpha-Lipoic acid reduced weight gain and improved the lipid profile in rats fed with high fat diet
Se, EY et al (2012). alpha-Lipoic acid reduced weight gain and improved the lipid profile in rats fed with high fat diet. Nutr Res Pract. 6(3):195-200. Assay: Cholesterol in rat plasma.
Effect of adrenomedullin gene delivery on insulin resistance in type 2 diabetic rats
Henein, HY et al (2011). Effect of adrenomedullin gene delivery on insulin resistance in type 2 diabetic rats. J Adv Res 2(1): 57-64. Assay: Cholesterol in rat serum.
Effect of adrenomedullin gene delivery on insulin resistance in type 2 diabetic rats
Hoda, Y. et al ( 2010). Effect of adrenomedullin gene delivery on insulin resistance in type 2 diabetic rats. J. Adv. Res. 2:57-64. Assay: Cholesterol in fish serum.
Effect of adrenomedullin gene delivery on insulin resistance in type 2 diabetic rats
Hoda, Y. et al ( 2010). Effect of adrenomedullin gene delivery on insulin resistance in type 2 diabetic rats. J. Adv. Res. 2:57-64. Assay: Cholesterol in rat serum.
Hu, M et al. (2010) Effect of prolonged starvation on body weight and blood-chemistry in two horseshoe crab species: Tac
Hu, M et al. (2010) Effect of prolonged starvation on body weight and blood-chemistry in two horseshoe crab species: Tachypleus tridentatus and Carcinoscorpius rotundicauda (Chelicerata: Xiphosura). J. Exp Marine Biol Ecology 395(1-2):112-119. Assay: Cholesterol in crab plasma.
Effect of prolonged starvation on body weight and blood-chemistry in two horseshoe crab species: Tachypleus tridentatus and Carcinoscorpius rotundicauda (Chelicerata: Xiphosura)
Hu, M et al. (2010). Effect of prolonged starvation on body weight and blood-chemistry in two horseshoe crab species: Tachypleus tridentatus and Carcinoscorpius rotundicauda (Chelicerata: Xiphosura). J Exp Marine Biol Ecol 395(1-2): 112-119. Assay: Cholesterol in Horseshoe crab plasma.
Hypercholesterolemia and myocardial function evaluated via tissue doppler imaging
Rubinstein J, et al (2009). Hypercholesterolemia and myocardial function evaluated via tissue doppler imaging. Cardiovasc Ultrasound. 7:56. Assay: Cholesterol in rabbit serum.
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