| Field | Specification |
|---|---|
| Detection Method | |
| Product Type | |
| Sample Type(s) | Biological samples, food, beverages, and the environment |
| Shipping | |
| Species | |
| Storage |
Overview
For formaldehyde determination in biological samples, food, beverages, and the environment. Fast and sensitive. Use of 50 µL sample. Linear detection range 1.2 to 500 µM Formaldehyde in 96-well plate assay. Convenient. The procedure. The assay uses OD565nm for signal readout. Compatible sample input includes Biological samples, food, beverages, and the environment. Typical stated assay timing is 30 min.
Key elements and design rationale
- Readout format: OD565nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Biological samples, food, beverages, and the environment, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 1.2μM for interpreting low-signal samples.
- Feature emphasis: Fast and sensitive. Use of 50 μL sample. Linear detection range 1.2 to 500 μM Formaldehyde in 96-well plate assay.
Additional feature notes highlight Convenient. The procedure involves adding a single working reagent and reading the absorbance after 30 minutes. Room temperature assay; High-throughput. “Add-mix-read” type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Available format information for this listing includes 100 Tests in 96-well plate.
Biological background
This product is centered on measurement of formaldehyde within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
FORMALDEHYDE(methanal) is the simplest aldehyde. It is widely employed in industry for a wide range of applications. Formaldehyde is also used as a disinfectant and is a commonly utilized tissue fixative and embalming agent. Formaldehyde is naturally present in all tissues and body fluids. Recently, it has been shown that some cancer types exhibit elevated formaldehyde levels. Increased formaldehyde concentration in urine has been associated with prostate and bladder cancer. Thus, measuring formaldehyde in urine can be a very useful tool when studying cancer.BioAssay Systems’ formaldehyde assay kit is based on formaldehyde dehydrogenase catalyzed oxidation of formaldehyde, which generates formate and NADH that reduces a formazan (MTT) dye. The intensity of the reduced MTT, measured at 565 nm, is directly proportional to formaldehyde concentration in the sample.
Detection method
Colorimetric (OD 565 nm).
Detection limit and analytical sensitivity
Reported detection limit: 1.2μM.
Procedures and timing
Stated procedure or timing information: 30 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.
Common research applications
- Quantify formaldehyde in biological samples, food, beverages by OD565 nm readout.
- Compare treatment or phenotype groups using matched biological samples, food, beverages handling.
- Monitor time-course or pre/post changes in biological samples, food, beverages across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
What additional materials are required, but not provided?
Pipetting devices, centrifuge tubes, clear flat-bottom 96-well plates (e.g. VWR cat# 82050-760), and plate reader.
What is the conversion for μM to ppb for formaldehyde?
1 μM equals 30 ppb formaldehyde.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
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