| Field | Specification |
|---|---|
| Mfr No | |
| Assay Time | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Food, beverage, and agricultural products |
| Shipping | |
| Species | |
| Storage |
Overview
For quantitative determination of histamine in food, beverage and agricultural products. The assay uses OD565nm for signal readout. Compatible sample input includes Food, beverage, and agricultural products. Typical stated assay timing is 30 min.
Key elements and design rationale
- Readout format: OD565nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Food, beverage, and agricultural products, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 2.5 µM for interpreting low-signal samples.
- Feature emphasis: Fast and sensitive. Use of 20 µL sample. Linear detection range 0.0025 – 1 mM (0.3 – 111 ppm) histamine in 96-well plate assay.
Additional feature notes highlight Convenient. The procedure involves adding a single working reagent and reading the absorbance after 30 minutes. Room temperature assay. No 37°C heater is needed; High-throughput. “Add-mix-read” type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Available format information for this listing includes 100 Tests.
Biological background
This product is centered on measurement of histamine within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
HISTAMINE (C5H9N3) is a biogenic amine naturally present in many foods and body cells in amounts without toxicological significance. It is also a contaminant that is mostly found in the Scombridae family fishes such as tuna and mackerel or other seafood products when improperly handled or stored. The consumption of foods containing high levels of histamine may lead to an allergy-like food poisoning known as scombroid poisoning.BioAssay Systems’ histamine assay kit is based on histamine dehydrogenase catalyzed oxidation of histamine in which the formed electron mediator reduces a formazan (MTT) reagent. The intensity of product color, measured at 565 nm is directly proportional to histamine concentration in the sample.
Detection method
Colorimetric (OD 565 nm).
Detection limit and analytical sensitivity
Reported detection limit: 2.5 µM.
Procedures and timing
Stated procedure or timing information: 30 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.
Common research applications
- Quantify histamine in food, beverage, and agricultural products by OD565 nm readout.
- Compare treatment or phenotype groups using matched food, beverage, and agricultural products handling.
- Monitor time-course or pre/post changes in food, beverage, and agricultural products across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
Does the assay kit work in particular species?
Our assays work in all species. None of our kits are species specific.
Do you offer free sample kits or discounts for testing?
Unfortunately, at this time we do not offer free sample kits. Customers must purchase assay kits for their own testing, on occasion we will offer discounts.
Does the histamine kit suitable for biological samples such as blood, urine, etc.?
Since most of the biological samples contain histamine under the kit detection limit, this kit is only suitable for food, beverage, and agricultural products.
How do I prepare the solid & liquid samples?
Fish or other solid samples: Weigh ˜1 g sample and grind to paste. Weigh ˜200mg paste sample and homogenize in 800 μL 0.05% acetic acid. May vortex or use handheld homogenzier to homogenize sample. If using blender to homogenize sample, just simply scale up the sample size and solvent in 1mg: 4 μL ratio (ex: 5 g samples in 20 mL acid). Centrifuge homogenate at 14,000 g for 10 min. Transfer the clear supernatant into a clean tube. It is prudent to run a pilot test of the sample at different dilutions. Choose a dilution with the readings in the detection range of the standard curve for further assays. Most samples can be stored at -80°C if not assayed immediately.
Cheese: Weigh ˜200 mg sample and homogenzie in 800 μL 0.05% acetic acid. Heat in heat bath at 60°C for 10mins. Centrifuge homogenate at 14,000 g for 10 min. Transfer the clear supernatant into a clean tube.
Liquid samples: Fish sauce or soy sauce: 1:5 dilution in H2O. Red wine or other alcoholic beverage: No dilution necessary.
Can I store unused reagents for future use?
Yes, unused reagents can be stored according to the assay protocol. Repeated freeze/thaw cycles of reagents should be avoided.
Do I need to use a standard or standard curve with each assay run?
Yes, it is highly recommended to run for each assay a standard curve, or where applicable, internal standard.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
Development of a histamine aptasensor for food safety monitoring
Dwidar, M., & Yokobayashi, Y. (2019). Development of a histamine aptasensor for food safety monitoring. Scientific Reports, 9(1), 16659. Assay: Histamine in tuna meat.
