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| Product Type | |
| Sample Type(s) | Cells |
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Overview
For quantitative fluorescent immunoenzymatic assay of ERK1/2 phosphorylation status in cultured cells. The assay uses Cell-Based ELISA (FL360/450nm, 530/585nm) for signal readout. Compatible sample input includes Cells. Typical stated assay timing is Assay takes 6.5 hrs, hands-on time 2.5 hrs.
Key elements and design rationale
- Readout format: Cell-Based ELISA (FL360/450nm, 530/585nm) supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Cells, which is useful when aligning matrix type with calibration and control design.
- Workflow timing: The listed assay time of Assay takes 6.5 hrs, hands-on time 2.5 hrs helps frame batch planning, replicate handling, and plate throughput.
- Feature emphasis: New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs).
Additional feature notes highlight Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis is necessary; Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Available format information for this listing includes 100 Tests.
Biological background
This product is centered on measurement of enzyfluo erk phosphorylation within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
Themitogen-activated protein kinase (MAPK/ERK) pathway plays a key role in cell proliferation, differentiation, and migration. Stimulation by mitogens eventually leads to phosphorylation of ERK1 (T202/Y204) and ERK2 (T185/Y187). The MAPK/ERK cascade presents many interesting drug targets for the development of cancer therapies. BioAssay Systems cell-based ELISA measures dually phosphorylated ERK1/2 in whole cells and normalizes the signal to the total protein content. This simple and efficient assay eliminates the need for cell lysate preparation and can be used to study kinase signaling and the effects of kinase inhibitors on cells. In this assay, cells are grown in 96-well plates and treated with ligands or drugs. Cells are then fixed and permeabilized in the wells. ERK1/2 phosphorylation (pERK) using a fluorescent ELISA followed by total protein measurement in each well.
Detection method
Cell-Based ELISA (FL360/450nm, 530/585nm).
Procedures and timing
Stated procedure or timing information: Assay takes 6.5 hrs, hands-on time 2.5 hrs.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.
Common research applications
- Quantify enzyfluo erk phosphorylation in cells by Cell-Based ELISA (FL360/450 nm, 530/585 nm) readout.
- Compare treatment or phenotype groups using matched cells handling.
- Monitor time-course or pre/post changes in cells across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
