EnzyFluo™ Glucose Uptake Assay Kit

Overview

For quantitative determination of glucose uptake in whole cells and evaluation of effects of ligands or drugs on glucose transport. The assay uses FL530/585nm for signal readout. Compatible sample input includes Cell culture. Typical stated assay timing is Approximately 2 hrs.

Key elements and design rationale

• Readout format: FL530/585nm supports plate-based signal acquisition and consistent comparison across matched samples.
• Sample compatibility: The stated sample scope includes Cell culture, which is useful when aligning matrix type with calibration and control design.
• Analytical range context: The supplied specifications include a stated detection limit of 0.1 µM for interpreting low-signal samples.
• Feature emphasis: Safe. No radioactive material is used.

Additional feature notes highlight Sensitive and Accurate. The detection limit of 0.1 µM and linearity up to 5 µM 2-DG6P; Simple and Convenient. Can be automated as a medium throughput assay for glucose transport in cells. Available format information for this listing includes 100 Tests.

Biological background

This product is centered on measurement of enzyfluo glucose uptake within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.

More details

Glucose Uptake has a variety of methods and transporters and depends upon the metabolic demand of the cell type and the availability of glucose. There are over ten different facilitated diffusion glucose transporters that transport glucose down its concentration gradient without ATP hydrolysis. In the kidneys, secondary active transport is used to uptake Glucose against its concentration gradient to ensure that very little glucose is excreted in the urine.BioAssay Systems fluorescent cell-based glucose uptake assay uses 2-deoxyglucose (2-DG), a widely used glucose analog because it can be taken up by glucose transporters and metabolized by endogenous hexokinase into 2-deoxyglucose 6-phosphate (2-DG6P). 2-DG6P accumulates intracellularly because it is not a suitable substrate for phosphoglucose isomerase, the next step in glycolysis. The cells are lysed, and excess NADP and glucose 6-phosphate dehydrogenase (G6PDH) are added to metabolize 2-DG6P and generate a molar equivalent amount of NADPH. The NADPH is then measured using a G6PDH recycling reaction to amplify the signal and generate a fluorescent signal measurable at λex/em = 530/585 nm proportional to the concentration of 2-DG6

Detection method

Fluorescent (FL 530/585 nm).

Detection limit and analytical sensitivity

Reported detection limit: 0.1 µM.

Procedures and timing

Stated procedure or timing information: Approximately 2 hrs.

Research relevance and current trends

• Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
• The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.
• Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.

Common research applications

• Quantify enzyfluo glucose uptake in cell culture by FL530/585 nm readout.
• Compare treatment or phenotype groups using matched cell culture handling.
• Monitor time-course or pre/post changes in cell culture across study conditions.

Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.

Notes for experimental interpretation

• Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
• Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.

For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.