| Field | Specification |
|---|---|
| Mfr No | |
| Assay Time | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Serum |
| Shipping | |
| Species | |
| Storage |
Overview
For quantitative determination of tryptophan and evaluation of drug effects on its metabolism. The assay uses FL530/585nm for signal readout. Compatible sample input includes Serum. Typical stated assay timing is 30 min.
Key elements and design rationale
- Readout format: FL530/585nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Serum, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 10 µM for interpreting low-signal samples.
- Feature emphasis: Fast and sensitive. Use of 10 µL sample. Linear detection range from 10µM to 400 µM tryptophan in a 96-well plate assay.
Additional feature notes highlight Convenient. The procedure involves adding a single working reagent and reading the fluorescence after 30 minutes. Room temperature assay. No 37°C heater is needed; High-throughput. “Add-mix-read” type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Available format information for this listing includes 100 Tests.
Biological background
This product is centered on measurement of enzyfluo tryptophan within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
TRYPTOPHAN is one of the eight essential amino acids that the body cannot synthesize and must be obtained through diet. Tryptophan is the biochemical precursor to the neurotransmitter serotonin, which has important roles in biological processes such as the regulation of appetite, sleep, and mood. Imbalances of serotonin have been linked to numerous mental health disorders. Tryptophan is also a precursor to the neurotransmitter melatonin, which is heavily involved in regulating the body’s sleep cycle. BioAssay Systems EnzyFluo™ Tryptophan Assay uses a coupled enzymatic reaction to determine the tryptophan concentration of a sample with the addition of a single working reagent. The fluorescence intensity at λex/em= 530/585 nm is directly proportional to the tryptophan concentration in the sample.
Detection method
Fluorescent (FL 530/585 nm).
Detection limit and analytical sensitivity
Reported detection limit: 10 µM.
Procedures and timing
Stated procedure or timing information: 30 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.
Common research applications
- Quantify enzyfluo tryptophan in serum by FL530/585 nm readout.
- Compare treatment or phenotype groups using matched serum handling.
- Monitor time-course or pre/post changes in serum across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
How do I store the kit?
This kit is shipped on ice. Upon receiving it, please keep the kit in the freezer (-20°C).
Does the kit work with urine samples?
Due to high levels of interference in urine samples, the assay cannot be used to detect tryptophan in urine.
There is a standard curve shown in the datasheet, why do I not construct one?
Yes, there is a standard curve in the datasheet; we include this to show the linear detection range of the kit for quantifying tryptophan. Due to interference matrices in serum and plasma, an internal standard must be run. The datasheet has detailed information on how to perform this and calculate tryptophan concentration from it.
I would like to construct a standard curve, how can I go about that?
It should be noted that this standard curve will not be accurate for determining tryptophan concentration in serum or plasma samples. However, if you wish to construct a standard curve, you may dilute the Tryptophan Standard in dH2O to concentrations within the kit’s detection range (e.g. 100µM, 50µM, 25µM, 0µM) and use these as samples for the fluorimetric (10µL) procedure. Add 100µL Working Reagents to these samples, incubate for 60 minutes, and read the RFU.
Can I store unused reagents for future use?
Yes, unused reagents can be stored according to the assay protocol. Repeated freeze/thaw cycles of reagents should be avoided. Working Reagent should be made fresh for each assay.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
