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The EOMA cell line, also known as EOMA endothelial cells, is derived from a spontaneously arising hemangioendothelioma in a mouse. This cell line is extensively used in research to study angiogenesis, the process of new blood vessel formation, which is critical in both normal physiological processes and in pathological conditions such as cancer, diabetic retinopathy, and rheumatoid arthritis. EOMA cells are characterized by their endothelial origin, displaying properties typical of endothelial cells, including the formation of capillary-like structures in vitro.
Researchers utilize the EOMA cell line to investigate the molecular and cellular mechanisms underlying angiogenesis. This includes studies on the role of various growth factors, signaling pathways, and the extracellular matrix in endothelial cell proliferation, migration, and tube formation. EOMA cells are particularly valuable in evaluating the effects of anti-angiogenic compounds, which are used in the treatment of cancer and other diseases involving abnormal blood vessel growth. These cells are also used in gene expression studies and in the development of therapeutic strategies targeting angiogenesis.
In addition to angiogenesis research, EOMA cells serve as a model for studying hemangioendothelioma, a rare vascular tumor, providing insights into tumor biology and the identification of potential therapeutic targets. By offering a reliable and reproducible in vitro system, the EOMA cell line significantly contributes to the understanding of vascular biology and the development of treatments for angiogenesis-related diseases.
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- Protein expression: angiotensin converting enzyme (ACE), thrombospondin, cathepsin L, endostatin, interleukin-6 (interleukin 6, IL-6)
- Antigen expression: CD31 +, vascular addressin +, CD45 (Ly5-T200) +
- Tumorigenic: Yes, in syngeneic mice
- cultureMedium: DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
- supplements: Supplement the medium with 10% FBS
- dissociationReagent: Accutase
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- fluidRenewal: 2 to 3 times per week
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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