| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Eph receptor B1+Eph receptor B2; EPH B1; EPH B2; Eph tyrosine kinase 2; Eph tyrosine kinase 3; EPHB1; EPHB2; Ephrin receptor EphB1; Ephrin receptor EphB2; Ephrin type B receptor 1; Ephrin type B receptor 2; EPHT 2; EPHT 3; ERK; Hek 5; Hek 6; Tyrosine protein kinase receptor EPH2; Tyrosine protein kinase receptor EPH3; EPHB1_HUMAN |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | KLH conjugated synthetic peptide derived from human EphB1+EphB2: 901-984/984 |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage |
Product Overview
EphB1/2 polyclonal antibody detects endogenous levels of EphB1/2 protein.
Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Cognate/functional ephrin ligands for this receptor include EFNB1, EFNB2 and EFNB3.
Validated Applications
| Application | Recommended Dilution |
|---|---|
| Western Blot (WB) | 1:500~1:2000 |
| Immunofluorescence (IF) | 1:100~1:500 |
| Immunohistochemistry (IHC) | 1:100~1:500 |
| ELISA | 1:5000~1:10000 |
Product Specifications
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Species Reactivity | Human, Mouse, Rat |
| Conjugate | Unconjugated |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Formulation | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| Molecular Weight | ~ 125 kDa |
| Modification | Unmodified |
| UniProt / Swiss-Prot | P54762 |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
Immunogen / Antigen
KLH conjugated synthetic peptide derived from human EphB1+EphB2: 901-984/984
Safety & Handling
For Research Use Only (RUO). Not for diagnostic or therapeutic use. Handle as a potential biohazard in accordance with good laboratory practices.
Related Products
Explore complementary antibodies, ELISA kits, and detection reagents at BioHippo.
This antibody has been validated for WB, IF, IHC, ELISA. Always refer to the product datasheet for recommended dilution ranges and appropriate positive control samples for each application type.
Validated reactivity has been confirmed for Human, Mouse, Rat. Cross-reactivity with additional species may exist based on sequence homology but has not been confirmed by the manufacturer. For unlisted species, we recommend running a preliminary validation experiment.
This antibody was raised in Rabbit. For indirect detection (WB, IHC, IF), select an anti-Rabbit secondary antibody that matches the isotype. For direct detection, check the variant selector for conjugated formats. Confirm secondary antibody compatibility before ordering.
KLH conjugated synthetic peptide derived from human EphB1+EphB2: 901-984/984
Please refer to the storage conditions on the product label and datasheet. As a general best practice: upon receipt, aliquot into single-use volumes to minimize freeze-thaw cycling, which can progressively reduce antibody performance and lead to aggregation.
Validated reactivity covers Human, Mouse, Rat samples. Performance can vary across different cell lines and tissue types depending on target antigen expression levels and epitope accessibility. We recommend verifying target expression in your specific model system using a known positive control before proceeding to experimental samples.
BioHippo offers customization and value-added services for qualified research programs:
- Bulk Pricing: Volume discounts available for orders of 5 or more units. Request a quote through the Contact Us page.
- Custom Conjugation: Select antibody products are available for custom conjugation to fluorophores, biotin, HRP, or other detection labels upon request. Minimum quantities apply.
- Custom Formats: Lyophilized or concentrated formats may be available for select products. Inquire before ordering.
- Technical Support: Our scientific team is available to assist with application optimization, protocol troubleshooting, and experimental design.
For customization inquiries, please use the Contact Us page or reach out to our support team directly.
