| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | Estrogen Receptor purified from cow uterus was used as the immunogen for the ER alpha antibody. The epitope of this mAb is mapped between amino acids 495-595. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
ER alpha Antibody / Estrogen Receptor alpha is an antibody targeting ER. Host: Mouse. Monoclonal. Clone: AER311. Format: Purified. Applications: FACS, IF.
Key elements and design rationale
- Target: ER.
- Immunogen: Estrogen Receptor purified from cow uterus was used as the immunogen for the ER alpha antibody. The epitope of this mAb….
- Species reactivity: Human.
- Localization: Nuclear.
- Conjugate: Unconjugated (no label listed).
Biological background (from provided description)
This mAb is specific to ER alpha and shows minimal cross-reaction with other members of the family. Epitope of this mAb is mapped between amino acids 495-595 of E/F domain. Estrogen Receptor is an important regulator of growth and differentiation in the mammary gland. Presence of Estrogen Receptor in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.
Common research applications
- IF/ICC: subcellular localization imaging with proper background controls.
Notes for experimental interpretation
- Account for isoforms, paralogs, and post-translational modifications when interpreting signal.
- Support specificity with biological controls (e.g., KO/KD where available) and technical controls (e.g., secondary-only/isotype).
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
