| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Blasticidin, Puromycin |
| Shipping | |
| Species |
Background
The estrogen response element (ERE) is a DNA sequence that mediates transcription driven by the estrogen receptor (ER), a ligand-activated nuclear receptor encoded by ESR1. Upon binding estrogens such as estradiol, ER dimerizes, translocates to the nucleus, and binds estrogen response elements to regulate genes controlling reproductive tissue development, bone density, and cardiovascular and metabolic physiology. ER signaling is a central driver of hormone-responsive breast cancer, where it remains a major therapeutic target. ERE-driven reporters give a quantitative readout of estrogen receptor transcriptional activity for endocrinology, steroid hormone, and cancer research.
Product Description & Applications
The ERE Reporter Lentivirus is a transcription factor reporter system that places a reporter gene under the control of tandem estrogen response elements coupled to a minimal promoter, giving a sensitive fluorescent or luminescent readout of estrogen receptor activation. Reporter options include EGFP, GFP, mCherry, RFP, firefly luciferase, and Renilla luciferase, with optional blasticidin or puromycin selection for stable polyclonal cell line generation. Particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and thawed cells.
Applications include monitoring ER signaling pathway activity, characterizing estrogens and anti-estrogens, and screening endocrine-active compounds in endocrinology and cancer research.
About This Product
This reporter lentivirus places a EGFP, Firefly Luc, mCherry, Renilla Luc, GFP, RFP, Luc reporter gene under the control of tandem consensus response elements specific for the Estrogen receptor (ER) signaling pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
Regulatory role for tumor suppressor REST on estrogen receptor (ESR1) expression and leiomyoma pathophysiology.
Bird SG, Gunewardena S, Cloud A, Ganeshkumar S, et al.
Journal of Biological Chemistry, 2026. DOI: 10.1016/j.jbc.2025.111017
Product(s) used: LTV-0046
Usage: ERE Reporter Lentivirus (LTV-0046-3S, MOI=1) transduced into MCF7 cells; firefly luciferase output used to measure estrogen receptor (ERα) transcriptional activity in leiomyoma pathophysiology under REST tumor suppressor regulation.
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