| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Reporter | |
| Selection Marker | Blasticidin, Hygromycin, Puromycin, Zeocin |
| Shipping | |
| Species |
Background
ERG (ETS-related gene) is a transcription factor of the ETS family that recognizes a core GGAA DNA-binding motif with preferences for specific flanking sequences. ERG regulates embryonic development, cell proliferation, differentiation, angiogenesis, inflammation, and apoptosis, and is an important regulator of endothelial and hematopoietic cell function. ERG often cooperates with the AP-1 transcription factor complex for optimal transcriptional activity. Chromosomal translocations involving ERG generate oncogenic fusion proteins, including TMPRSS2-ERG in prostate cancer, EWS-ERG in Ewing sarcoma, and FUS-ERG in acute myeloid leukemia, making ERG transcriptional activity an important readout in oncology and vascular biology research.
Product Description & Applications
The ERG Reporter Lentivirus places a reporter gene under the control of consensus ERG DNA-binding elements that include an AP-1-like motif, providing a sensitive readout of ERG transcriptional activity in human and mouse cells. A broad range of reporter options is available, including fluorescent (GFP, EGFP, d2GFP, BFP2, mCherry, RFP), luminescent (firefly, Renilla, and Gaussia luciferase), and combined formats, with blasticidin, hygromycin, puromycin, or zeocin selection.
The particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and cryopreserved cultures. Detection is possible by fluorescence microscopy, flow cytometry, or luminometry. Applications include monitoring ERG activity in cancer and endothelial models.
About This Product
This reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the ERG transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
