| Field | Specification |
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| Immunogen | A synthesized peptide derived from human ERP27 was used as the immunogen for the ERP27 antibody. |
| Isotype | |
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Overview
ERP27 Antibody / Endoplasmic reticulum resident protein 27 / TXNDC13 is a anti-ERP27 Rabbit antibody Recombinant Rabbit Monoclonal clone 32E24 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: ERP27
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 32E24, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, ICC, IF
Biological background
Endoplasmic reticulum resident protein 27 is localized to the lumen of the endoplasmic reticulum, where it interacts with nascent polypeptides and folding intermediates. Although it lacks enzymatic activity, ERP27 binds to unfolded proteins, potentially presenting them to catalytic PDIs for isomerization. Research using ERP27 antibody has demonstrated that it contributes to quality control of protein folding, ensuring only properly folded proteins proceed through the secretory pathway. By acting as a chaperone like molecule, ERP27 complements enzymatic PDIs in maintaining proteostasis.
ERP27 is upregulated during conditions of endoplasmic reticulum stress, such as accumulation of unfolded proteins or perturbations in calcium homeostasis. Studies with ERP27 antibody have shown that its expression is regulated by the unfolded protein response, linking it to pathways that restore folding capacity and reduce stress. Dysregulation of ERP27 may contribute to diseases characterized by endoplasmic reticulum stress, including diabetes, neurodegeneration, and cancer. Understanding ERP27 expression and function helps clarify how cells cope with misfolded proteins and maintain secretory pathway fidelity.
In cancer, altered expression of endoplasmic reticulum chaperones influences tumor survival and progression. Research with ERP27 antibody has indicated that increased expression may provide tumor cells with resilience against proteotoxic stress, while reduced levels may impair secretory function. Its role in regulating folding efficiency positions ERP27 as an emerging factor in tumor biology and therapeutic resistance. Beyond cancer, ERP27 contributes to cellular stress adaptation across a wide range of tissues, including secretory cells of the pancreas and liver.
ERP27 antibody is widely applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting demonstrates induction under stress conditions, immunohistochemistry shows distribution in secretory tissues, and immunofluorescence confirms localization to the endoplasmic reticulum. These applications make ERP27 antibody valuable for research into protein folding, stress responses, and secretory pathway regulation.
By providing validated ERP27 antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
