| Field | Specification |
|---|---|
| Target | |
| Alternative names | esxA; SAS0258Type VII secretion system extracellular protein A; Ess extracellular protein A |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Recombinant Staphylococcus aureus ESAT-6 secretion system extracellular protein A protein (1-97AA) |
| Conjugate | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Purification | |
| Form | Liquid |
| Storage | |
| Storage buffer | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
This polyclonal anti-esxA antibody raised in Rabbit, conjugated with FITC. Designed to detect esxA in Staphylococcus aureus for others research.
Key elements and design rationale
- Immunogen: Recombinant Staphylococcus aureus ESAT-6 secretion system extracellular protein A protein (1-97AA) — determines epitope; confirm sample prep compatibility.
- Host (Rabbit): Requires anti-rabbit-IgG secondary matching isotype IgG.
- Polyclonal: Multiple epitopes = robust signal; lot-to-lot variability — include controls.
- Isotype (IgG): Match secondary, Protein A/G, and isotype controls to IgG.
- Purification (Protein G purification): Enriches Ig classes; reduces non-specific background.
- FITC: Direct fluorescence (Ex490/Em525); protect from light; use antifade.
Biological background
esxA (also: esxA, SAS0258Type VII secretion system extracellular protein A, Ess extracellular protein A) — studied in Staphylococcus aureus (strain MSSA476) systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current esxA annotation relevant to others.
Common research applications
- Refer to product documentation for confirmed application data.
Notes for experimental interpretation
- Isotype controls: IgG from Rabbit at equivalent concentration.
- Cross-reactivity: Polyclonal: validate with KO/KD controls.
- Matrix effects: Verify dilution linearity in your sample matrix.
- Reactivity: Confirmed for Staphylococcus aureus; extrapolation requires validation.
This product is a polyclonal antibody purified from rabbit antiserum.
esxA is a protein studied in Staphylococcus aureus biology. This polyclonal antibody raised in Rabbit detects esxA in immunoassay applications, IgG isotype.
Reported reactive against Staphylococcus aureus. Immunogen from Staphylococcus aureus (strain MSSA476). Cross-reactivity with untested species requires empirical confirmation.
Application data not specified. Consult datasheet or contact BioHippo support.
FITC (Ex490/Em525): direct fluorescence; protect from light; use antifade for sections.
(1) Isotype control: IgG from Rabbit; (2) Positive control: lysate/cell line expressing esxA; (3) Negative control: siRNA KD or KO; (4) Dilution linearity in your sample matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
