| Field | Specification |
|---|---|
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| Activity | |
| Cas No. | |
| Concentration | |
| Form | Lyophilized powder. |
| Product Type | |
| Purity | |
| Reconstitution | |
| Shipping | |
| Solubility | Water. Centrifuge all product preparations before use (10000 x g for 1 min). |
| Source | Synthetic |
| Storage | |
| Target |
Product details
- Chemical name: Tetrasodium;(6E)-4-amino-6-[[4-[4-[(2E)-2-(8-amino-1-oxo-5,7-disulfonatonaphthalen-2-ylidene)hydrazinyl]-3-methylphenyl]-2-methylphenyl]hydrazinylidene]-5-oxonaphthalene-1,3-disulfonate.
- Also known as: Direct Blue 53, Azovan Blue, Evan's Blue, Diazobleu, Evablin, Dye Evans Blue, Evans Blue Dye, Geigy-blau 536, Diamine Sky Blue FF
- CAS number: 314-13-6
- Molecular formula: C34H24N6Na4O14S4.
- Purity: >90%
- Concentration: 50 nM - 100 µM.
- Form: Lyophilized powder.
- Solubility: Water. Centrifuge all product preparations before use (10000 x g for 1 min).
- Reconstitution: Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. For long-term storage of diluted solutions, we recommend adding 0.1% BSA. Repeat freeze-thawing may result in loss of activity.
- Shipping: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
- Target: AMPA, Kainate, P2X receptors
- Source: Synthetic
- Activity: Evans Blue tetrasodium salt blocks glutamate receptors with IC50s 150 nM and 220 nM for kainate-type GluR6 and AMPA-type GluR11.
- Alternative names: Direct Blue 53, Azovan Blue, Evan's Blue, Diazobleu, Evablin, Dye Evans Blue, Evans Blue Dye, Geigy-blau 536, Diamine Sky Blue FF
Scientific background
Glutamate is the major excitatory neurotransmitter in the mammalian central nervous system and detailed knowledge of the properties of glutamate receptors is essential in understanding excitatory synaptic transmission in the brain. Glutamatergic transmission at excitatory synapses is mediated by three different classes of receptor-gated ion channels: AMPA receptors (α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid), Kainate receptors and NMDA receptors (N-methyl-D-aspartate)1.The biphenyl derivative of 1,3-naphthalene disulfonic acid, known as Evans Blue tetrasodium salt, was found to reduce desensitization and partially block the peak amplitudes of non-NMDA-receptor mediated currents in rat thalamic neurons2, and to block kainate evoked currents of heterologously expressed recombinant AMPA receptors containing either subunit GluR1 or GluR2 (IC50 values are 220 µM)3. Evans Blue has been an important tool in many physiological and clinical investigations because of its high affinity for plasma albumin4.
Lead time: 1-2 Business Days
Country of origin: Israel/IL
Applications key
Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.
Bioassay tested: Yes
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- Add-on labels & handles: D/¹³C/¹⁵N isotopes (LC-MS/internal standards), azide/alkyne or other functional handles for conjugation
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